Construct: ORF ccsbBroadEn_08267
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010203.1_s300c1, BRDN0000390578
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLF3 (51274)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51274 | KLF3 | Kruppel like factor 3 | NM_016531.6 | 99.9% | 99.7% | 914A>G |
| 2 | human | 51274 | KLF3 | Kruppel like factor 3 | XM_017008279.2 | 92.6% | 92.4% | 0_1ins75;839A>G |
| 3 | human | 51274 | KLF3 | Kruppel like factor 3 | XR_925142.1 | 59.2% | 1_332del;1028_1106del;1268_1269ins178 | |
| 4 | human | 51274 | KLF3 | Kruppel like factor 3 | XR_001741235.2 | 30.1% | (many diffs) | |
| 5 | mouse | 16599 | Klf3 | Kruppel-like factor 3 (basic) | NM_008453.5 | 86.5% | 95% | (many diffs) |
| 6 | mouse | 16599 | Klf3 | Kruppel-like factor 3 (basic) | XM_006503751.3 | 86.5% | 95% | (many diffs) |
| 7 | mouse | 16599 | Klf3 | Kruppel-like factor 3 (basic) | XR_001784628.1 | 17.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1101
- ORF length:
- 1035
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCT CATGTTTGAC CCAGTTCCTG TCAAGCAAGA GGCCATGGAC CCTGTCTCAG 121 TGTCATACCC ATCTAATTAC ATGGAATCCA TGAAGCCTAA CAAGTATGGG GTCATCTACT 181 CCACACCATT GCCTGAGAAG TTCTTTCAGA CCCCAGAAGG TCTGTCGCAC GGAATACAGA 241 TGGAGCCAGT GGACCTCACG GTGAACAAGC GGAGTTCACC CCCTTCGGCT GGGAATTCGC 301 CCTCCTCTCT GAAGTTCCCG TCCTCACACC GGAGAGCCTC GCCTGGGTTG AGCATGCCTT 361 CTTCCAGCCC ACCGATAAAA AAATACTCAC CCCCTTCTCC AGGCGTGCAG CCCTTCGGCG 421 TGCCGCTGTC CATGCCACCA GTGATGGCAG CTGCCCTCTC GCGGCATGGA ATACGGAGCC 481 CGGGGATCCT GCCCGTCATC CAGCCGGTGG TGGTGCAGCC CGTCCCCTTT ATGTACACAA 541 GTCACCTCCA GCAGCCTCTC ATGGTCTCCT TATCGGAGGA GATGGAAAAT TCCAGTAGTA 601 GCATGCAAGT ACCTGTAATT GAATCATATG AGAAGCCTAT ATCACAGAAA AAAATTAAAA 661 TAGAACCTGG GATCGAACCA CAGAGGACAG ATTATTATCC TGAAGAAATG TCACCCCCCT 721 TAATGAACTC AGTGTCCCCC CCGCAAGCAT TGTTGCAAGA GAATCACCCT TCGGTCATCG 781 TGCAGCCTGG GAAGAGACCT TTACCTGTGG AATCCCCGGA TACTCAAAGG AAGCGGAGGA 841 TACACAGATG TGATTATGAT GGATGCAACA AAGTGTACAC TAAAAGCTCC CACTTGAAAG 901 CACACAGAAG AACACACACA GGAGAAAAAC CCTACAAATG TACATGGGAA GGGTGCACAT 961 GGAAGTTTGC TCGGTCTGGT GAACTAACAA GACATTTCCG AAAACATACT GGAATCAAAC 1021 CTTTCCAGTG CCCGGACTGT GACCGCAGCT TCTCCCGTTC TGACCATCTT GCCCTCCATA 1081 GGAAACGCCA CATGCTAGTC TGCCCAACTT TCTTGTACAA AGTtggcatt ataagaaagc 1141 attgcttatc aatttgttgc aacgaac