Construct: ORF ccsbBroadEn_08448
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004324.1_s300c1, BRDN0000394739
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RMND1 (55005)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55005 | RMND1 | required for meiotic nuclea... | NM_017909.4 | 99.9% | 99.7% | 395C>N |
| 2 | human | 55005 | RMND1 | required for meiotic nuclea... | XR_002956288.1 | 69.3% | (many diffs) | |
| 3 | human | 55005 | RMND1 | required for meiotic nuclea... | XR_001743503.2 | 68.1% | 1_168del;563C>N;1516_1976del | |
| 4 | human | 55005 | RMND1 | required for meiotic nuclea... | NM_001271937.1 | 62.1% | 62.1% | 0_1ins510 |
| 5 | human | 55005 | RMND1 | required for meiotic nuclea... | XM_005267040.4 | 53% | 53% | 0_1ins633 |
| 6 | human | 55005 | RMND1 | required for meiotic nuclea... | XM_017010988.2 | 53% | 53% | 0_1ins633 |
| 7 | mouse | 66084 | Rmnd1 | required for meiotic nuclea... | XM_006512482.3 | 46.4% | 49.8% | (many diffs) |
| 8 | mouse | 66084 | Rmnd1 | required for meiotic nuclea... | XM_017314057.1 | 39.7% | 43.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1416
- ORF length:
- 1347
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GCCAGCCACA CTCCTCAGAG CCGTGGCCAG ATCTCATCAT ATATTATCAA 121 AAGCACATCA GTGCCGAAGA ATCGGTCATC TAATGTTAAA ACCACTTAAG GAATTTGAAA 181 ATACAACATG CAGCACACTG ACAATACGTC AAAGCTTGGA TTTGTTCCTT CCTGATAAAA 241 CAGCTAGTGG TTTGAATAAG TCTCAGATCC TGGAAATGAA CCAAAAAAAG TCAGATACCA 301 GCATGCTGTC TCCATTAAAT GCTGCTCGTT GCCAAGATGA AAAGGCACAC CTTCCAACCA 361 TGAAATCCTT TGGTACTCAC AGGAGAGTGA CCCACAAACC AAATCTGTTG GGTTCTAAAT 421 GGTTTATAAA AATATTAAAG AGGCATTTCT CATCTGTATC AANGGAAACA TTTGTTCCAA 481 AACAAGACTT CCCACAGGTG AAGAGACCAC TAAAAGCATC CAGGACCAGA CAGCCATCCA 541 GGACCAACCT TCCAGTTCTG TCTGTGAACG AGGACCTAAT GCACTGCACA GCATTTGCAA 601 CGGCAGATGA GTATCATCTG GGAAATCTGT CTCAAGATCT GGCCTCCCAC GGATATGTTG 661 AAGTAACAAG CTTGCCTAGA GATGCAGCAA ATATTTTGGT GATGGGTGTG GAAAATTCTG 721 CAAAAGAAGG TGATCCTGGA ACAATATTCT TCTTCAGGGA AGGAGCTGCT GTGTTTTGGA 781 ATGTGAAAGA CAAAACTATG AAGCATGTGA TGAAAGTTCT AGAAAAACAT GAAATTCAGC 841 CCTATGAAAT CGCACTGGTA CACTGGGAAA ATGAAGAACT TAACTACATA AAAATAGAGG 901 GACAGTCAAA ACTTCACAGG GGGGAAATCA AGTTAAATTC AGAGCTGGAT TTAGATGATG 961 CCATTCTAGA GAAGTTTGCT TTCTCCAATG CTCTATGCCT TTCTGTAAAA CTGGCAATTT 1021 GGGAAGCATC ACTGGATAAA TTTATTGAAT CTATTCAGTC AATTCCTGAG GCTTTAAAAG 1081 CTGGGAAGAA AGTGAAACTA TCTCATGAAG AAGTTATGCA GAAAATCGGT GAACTCTTTG 1141 CTCTAAGGCA CCGTATAAAC TTGAGTTCAG ACTTCCTGAT TACTCCTGAT TTCTACTGGG 1201 ACAGAGAAAA CCTGGAAGGA CTTTACGATA AAACGTGTCA ATTCCTTAGC ATTGGCCGAA 1261 GAGTTAAGGT CATGAATGAA AAACTTCAGC ACTGCATGGA ACTAACAGAT CTAATGCGGA 1321 ATCACCTGAA TGAGAAGAGG GCACTCCGCT TGGAGTGGAT GATTGTCATC CTCATTACCA 1381 TAGAGGTAAT GTTTGAGCTG GGACGAGTAT TTTTCTTGCC AACTTTCTTG TACAAAGTtg 1441 gcattataag aaagcattgc ttatcaattt gttgcaacga ac