Construct: ORF ccsbBroadEn_08669

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005509.1_s300c1, BRDN0000391507
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PMEPA1 (56937)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56937 PMEPA1 prostate transmembrane prot... NM_199169.3 99.6% 99.6% 118C>T;336A>G;354A>G
2 human 56937 PMEPA1 prostate transmembrane prot... NM_001255976.2 96.9% 96.5% (many diffs)
3 human 56937 PMEPA1 prostate transmembrane prot... NM_199170.3 93.6% 93.6% (many diffs)
4 human 56937 PMEPA1 prostate transmembrane prot... NM_199171.2 93.6% 93.6% (many diffs)
5 human 56937 PMEPA1 prostate transmembrane prot... NM_020182.5 87.4% 86.7% (many diffs)
6 mouse 65112 Pmepa1 prostate transmembrane prot... NM_022995.3 74.9% 78% (many diffs)
7 mouse 65112 Pmepa1 prostate transmembrane prot... XM_006499991.3 65.6% 69.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
825
ORF length:
756
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGCGGAGCTG GAGTTTGTTC AGATCATCAT CATCGTGGTG GTGATGATGG
121TGATGGTGGT GGTGATCACG TGCCTGCTGA GCCACTACAA GCTGTCTGCA CGGTCCTTCA
181TCAGCTGGCA CAGCCAGGGG CGGAGGAGAG AAGATGCCCT GTCCTCAGAA GGATGCCTGT
241GGCCCTCGGA GAGCACAGTG TCAGGCAACG GAATCCCAGA GCCGCAGGTC TACGCCCCGC
301CTCGGCCCAC CGACCGCCTG GCCGTGCCGC CCTTCGCCCA GCGGGAGCGC TTCCACCGCT
361TCCAGCCCAC CTATCCGTAC CTGCAGCACG AGATCGACCT GCCGCCCACC ATCTCGCTGT
421CGGACGGGGA GGAGCCCCCA CCCTACCAGG GCCCCTGCAC CCTCCAGCTT CGGGACCCCG
481AGCAGCAGCT GGAACTGAAC CGGGAGTCGG TGCGCGCACC CCCAAACAGA ACCATCTTCG
541ACAGTGACCT GATGGATAGT GCCAGGCTGG GCGGCCCCTG CCCCCCCAGC AGTAACTCGG
601GCATCAGCGC CACGTGCTAC GGCAGCGGCG GGCGCATGGA GGGGCCGCCG CCCACCTACA
661GCGAGGTCAT CGGCCACTAC CCGGGGTCCT CCTTCCAGCA CCAGCAGAGC AGTGGGCCGC
721CCTCCTTGCT GGAGGGGACC CGGCTCCACC ACACACACAT CGCGCCCCTA GAGAGCGCAG
781CCATCTGGAG CAAAGAGAAG GATAAACAGA AAGGACACCC TCTCTTGCCA ACTTTCTTGT
841ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)