Construct: ORF ccsbBroadEn_08811

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014546.1_s300c1, BRDN0000390289
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NSUN3 (63899)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 63899 NSUN3 NOP2/Sun RNA methyltransfer... NM_022072.5 99.9% 99.7% 884C>T
2 human 63899 NSUN3 NOP2/Sun RNA methyltransfer... XM_024453699.1 58.3% 56.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1086
ORF length:
1020
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaCTTTTG TACAAAAAAG
61TTGGCATGCT GACCCAGCTG AAAGCAAAAT CAGAGGGGAA GCTTGCAAAA CAGATTTGCA
121AAGTTGTGTT GGATCATTTT GAAAAACAGT ATTCCAAAGA ACTCGGAGAT GCCTGGAATA
181CAGTAAGGGA GATACTAACA TCTCCATCAT GCTGGCAATA TGCTGTCCTG CTTAACCGAT
241TCAATTATCC TTTTGAACTG GAAAAGGATT TACATTTGAA GGGCTATCAC ACACTCTCTC
301AGGGATCTTT ACCCAACTAT CCTAAATCAG TGAAGTGTTA CCTTAGCAGA ACTCCGGGCC
361GAATCCCTTC AGAAAGACAC CAAATTGGAA ACCTGAAAAA ATATTATCTC CTAAATGCTG
421CTTCTCTTCT CCCAGTGTTG GCTCTGGAAT TAAGGGATGG GGAGAAGGTT CTGGATCTCT
481GTGCTGCTCC TGGAGGGAAA TCAATAGCTC TGCTGCAGTG TGCTTGTCCA GGTTATCTTC
541ATTGTAATGA ATATGATAGT CTGAGATTGA GGTGGCTAAG GCAGACGTTG GAATCTTTCA
601TCCCACAGCC TTTGATAAAT GTAATTAAAG TGTCTGAATT GGATGGCAGA AAAATGGGAG
661ATGCCCAGCC TGAAATGTTT GACAAGGTGT TAGTGGATGC TCCGTGTTCA AATGATCGAA
721GCTGGTTGTT TTCTTCTGAC TCTCAGAAGG CATCCTGTAG GATAAGTCAA AGGAGGAATT
781TGCCTCTTCT ACAGATAGAG CTGTTAAGGT CTGCAATTAA GGCCTTACGT CCTGGAGGGA
841TACTTGTATA CTCTACATGC ACGCTTTCCA AGGCAGAAAA TCAAGATGTG ATCAGTGAAA
901TTTTAAACTC CCACGGTAAC ATCATGCCTA TGGACATTAA AGGAATAGTA AGGACTTGCT
961CCCACGACTT CACATTTGCT CCCACTGGCC AGGAATGTGG GCTCTTAGTG ATTCCAGATA
1021AGGGCAAAGC CTGGGGCCCA ATGTATGTAG CCAAATTGAA GAAATCATGG AGCACAGGAA
1081AATGGTGCCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt
1141gttgcaacga ac

Download FASTA (ORF) (Full)