Construct: ORF ccsbBroadEn_09277
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005601.1_s300c1, BRDN0000397432
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MVB12B (89853)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89853 | MVB12B | multivesicular body subunit... | NM_001011703.3 | 99.8% | 100% | 135G>A |
| 2 | human | 89853 | MVB12B | multivesicular body subunit... | XM_017015278.2 | 90.6% | 90.5% | 135G>A;663_729delinsG |
| 3 | human | 89853 | MVB12B | multivesicular body subunit... | XM_017015277.2 | 82.9% | 83% | 135G>A;664_798del |
| 4 | human | 89853 | MVB12B | multivesicular body subunit... | XR_929872.3 | 78.9% | 1_22del;157G>A;686_838del | |
| 5 | human | 89853 | MVB12B | multivesicular body subunit... | NM_033446.3 | 69.1% | 69.2% | 135G>A;664_957del |
| 6 | human | 89853 | MVB12B | multivesicular body subunit... | XM_017015275.2 | 65.4% | 65.5% | 135G>A;664_1011del |
| 7 | human | 89853 | MVB12B | multivesicular body subunit... | XM_005252297.1 | 62.3% | 59.8% | (many diffs) |
| 8 | human | 89853 | MVB12B | multivesicular body subunit... | XM_017015276.1 | 59% | 56.6% | (many diffs) |
| 9 | human | 89853 | MVB12B | multivesicular body subunit... | XM_024447711.1 | 56.2% | 56.3% | 0_1ins93;42G>A;571_918del |
| 10 | human | 89853 | MVB12B | multivesicular body subunit... | XM_017015279.1 | 32% | 32% | 0_1ins339;325_672del |
| 11 | mouse | 72543 | Mvb12b | multivesicular body subunit... | XM_006498373.1 | 80.7% | 87.2% | (many diffs) |
| 12 | mouse | 72543 | Mvb12b | multivesicular body subunit... | XM_011239194.2 | 76.2% | 82.5% | (many diffs) |
| 13 | mouse | 72543 | Mvb12b | multivesicular body subunit... | XM_006498372.1 | 74.7% | 80.9% | (many diffs) |
| 14 | mouse | 72543 | Mvb12b | multivesicular body subunit... | XM_006498370.1 | 64.4% | 69.8% | (many diffs) |
| 15 | mouse | 72543 | Mvb12b | multivesicular body subunit... | NM_175184.4 | 61.6% | 66.7% | (many diffs) |
| 16 | mouse | 72543 | Mvb12b | multivesicular body subunit... | XM_006498369.2 | 61.4% | 66.5% | (many diffs) |
| 17 | mouse | 72543 | Mvb12b | multivesicular body subunit... | XM_006498367.3 | 54.3% | 58.8% | (many diffs) |
| 18 | mouse | 72543 | Mvb12b | multivesicular body subunit... | XM_006498371.3 | 52.7% | 57.8% | (many diffs) |
| 19 | mouse | 72543 | Mvb12b | multivesicular body subunit... | XM_006498368.3 | 49.7% | 51.3% | (many diffs) |
| 20 | mouse | 72543 | Mvb12b | multivesicular body subunit... | XM_017319281.1 | 45% | 48.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 729
- ORF length:
- 663
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAG AAGCTGCTTC TGCGTGAGAC GGAGCCGGGA CCCGCCGCCG CCGCAGCCAC 121 CGCCGCCGCC GCCCCAGCGG GGAACAGACC AGTCCACCAT GCCTGAAGTC AAAGACCTCT 181 CAGAAGCCTT GCCAGAAACA TCAATGGATC CCATCACGGG AGTCGGGGTG GTGGCTTCTC 241 GGAACCGAGC CCCGACAGGC TATGACGTAG TTGCACAGAC AGCAGATGGT GTGGATGCTG 301 ACCTCTGGAA AGACGGCTTA TTTAAATCCA AGGTTACCAG ATACCTGTGT TTCACAAGAT 361 CATTTTCCAA AGAAAATAGT CATCTGGGGA ACGTGTTAGT AGATATGAAG CTCATTGACA 421 TCAAGGACAC ACTGCCTGTG GGCTTCATCC CAATTCAGGA GACGGTGGAC ACACAGGAAG 481 TGGCTTTTAG GAAGAAGAGG CTGTGCATTA AATTTATTCC ACGGGATTCA ACGGAAGCTG 541 CGATTTGTGA CATTCGGATC ATGGGCCGGA CCAAGCAGGC CCCGCCTCAG TACACGTTTA 601 TTGGGGAACT GAACAGCATG GGGATCTGGT ATCGAATGGG CAGAGTACCA AGAAATCATG 661 ACTCATCTCA ACCCACAACG CCTTCCCAGT CATCAGCTGC CTCCACCCCA GCCCCCAACC 721 TTCCCAGGTG CCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa 781 tttgttgcaa cgaac