Construct: ORF ccsbBroadEn_09850

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005571.1_s300c1, BRDN0000387289
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MRPL21 (219927)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 219927 MRPL21 mitochondrial ribosomal pro... NM_181514.2 98% 98% 0_1insATGGCGGCGGCC
2 human 219927 MRPL21 mitochondrial ribosomal pro... XR_247190.4 80.4% (many diffs)
3 human 219927 MRPL21 mitochondrial ribosomal pro... XM_005273823.4 66.5% 64% (many diffs)
4 human 219927 MRPL21 mitochondrial ribosomal pro... NM_181515.2 57.4% 57.4% 0_1ins267
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
693
ORF length:
627
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GGCGGCCATG GCAGCATCTT CCCTGACGGT CACCTTAGGG CGGCTGGCGT
121CCGCGTGCAG CCACAGCATC CTGAGACCTT CGGGGCCCGG AGCAGCCTCC CTTTGGTCTG
181CTTCTCGAAG GTTCAATTCA CAGAGCACTT CATATCTACC AGGATATGTT CCTAAAACAT
241CCCTGAGTTC ACCACCTTGG CCAGAAGTTG TTCTGCCAGA CCCAGTTGAG GAGACCAGAC
301ACCATGCAGA GGTCGTGAAG AAGGTGAATG AGATGATCGT CACGGGGCAG TATGGCAGGC
361TCTTTGCCGT GGTGCACTTT GCCAGCCGCC AGTGGAAGGT GACCTCTGAA GACCTGATCT
421TAATTGGAAA TGAACTAGAC CTTGCGTGTG GAGAGAGAAT TCGACTGGAG AAGGTCCTGC
481TGGTTGGGGC AGACAACTTC ACGCTGCTTG GCAAGCCACT CCTCGGAAAG GATCTTGTTC
541GAGTAGAAGC CACAGTCATT GAAAAGACAG AATCATGGCC AAGAATCATT ATGAGATTCA
601GGAAAAGGAA AAACTTCAAG AAGAAAAGAA TCGTCACGAC CCCGCAGACT GTCCTCCGGA
661TAAACAGCAT TGAGATTGCT CCGTGTTTGT TGTGCCTAAC TTTCTTGTAC AAAGTtggca
721ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)