Construct: ORF ccsbBroadEn_09851

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008446.1_s300c1, BRDN0000457119
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TPCN2 (219931)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 219931 TPCN2 two pore segment channel 2 NM_139075.4 99.7% 99.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2325
ORF length:
2256
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGCGGAACCC CAGGCGGAGT CGGAGCCCCT GCTGGGCGGG GCCCGCGGCG
121GTGGCGGCGA CTGGCCGGCG GGGCTGACCA CTTACCGCAG CATCCAAGTC GGCCCTGGTG
181CCGCGGCCAG GTGGGACCTC TGCATTGATC AGGCTGTGGT CTTCATCGAA GATGCTATTC
241AGTACCGCTC CATCAACCAC CGGGTGGATG CCAGCTCGAT GTGGCTTTAC CGACGGTATT
301ACTCGAACGT ATGCCAACGG ACTTTGAGCT TCACCATCTT CTTGATCCTG TTTTTGGCTT
361TTATCGAGAC CCCATCCTCA CTCACCAGCA CGGCGGACGT GCGCTACCGC GCTGCCCCCT
421GGGAGCCGCC CTGCGGCCTG ACCGAGAGTG TCGAGGTGCT CTGCCTGCTG GTCTTTGCGG
481CCGACCTCTC TGTGAAGGGT TACCTGTTCG GGTGGGCCCA TTTCCAGAAA AACCTTTGGC
541TGCTGGGCTA CCTCGTGGTG CTGGTGGTGT CTCTGGTGGA CTGGACCGTG TCCCTGAGTC
601TCGTGTGTCA TGAGCCCCTG CGGATCCGCC GGCTTCTCCG TCCCTTCTTC CTGCTGCAGA
661ACTCCTCTAT GATGAAGAAG ACCTTGAAAT GCATCCGCTG GTCGCTGCCG GAAATGGCCA
721GCGTCGGGCT GCTGCTGGCC ATCCACCTGT GCCTCTTCAC CATGTTCGGA ATGCTGCTGT
781TCGCTGGTGG GAAGCAGGAT GATGGGCAGG ACAGGGAGAG GCTGACCTAC TTCCAGAACC
841TGCCTGAGTC TCTGACTTCC CTCCTGGTGC TGCTGACCAC GGCCAACAAC CCCGATGTGA
901TGATTCCTGC GTATTCCAAG AACCGGGCCT ATGCCATCTT CTTCATAGTC TTCACTGTGA
961TAGGAAGCCT GTTTCTGATG AACCTGCTGA CAGCCATCAT CTACAGTCAG TTCCGGGGCT
1021ACCTGATGAA ATCTCTCCAG ACCTCGCTGT TTCGGAGGCG GCTGGGAACC CGGGCTGCCT
1081TTGAAGTCCT ATCCTCCATG GTGGGGGAGG GAGGAGCCTT CCCTCAGGCA GTTGGGGTGA
1141AGCCCCAGAA CTTGCTGCAG GTGCTTCAGA AGGTCCAGCT GGACAGCTCC CACAAACAGG
1201CCATGATGGA GAAGGTGCGT TCCTACGGCA GTGTTCTGCT GTCAGCTGAG GAGTTTCAGA
1261AGCTCTTCAA CGAGCTTGAC AGAAGTGTGG TTAAAGAGCA CCCGCCGAGG CCCGAGTACC
1321AGTCTCCGTT TCTGCAGAGC GCCCAGTTCC TCTTCGGCCA CTACTACTTT GACTACCTGG
1381GGAACCTCAT CGCCCTGGCA AACCTGGTGT CCATTTGCGT GTTCCTGGTG CTGGATGCAG
1441ATGTGCTGCC TGCTGAGCGT GATGACTTCA TCCTGGGGAT TCTCAACTGC GTCTTCATTG
1501TGTACTACCT GTTGGAGATG CTGCTCAAGG TCTTTGCCCT GGGCCTGCGA GGGTACCTGT
1561CCTACCCCAG CAACGTGTTT GACGGGCTCC TCACCGTTGT CCTGCTGGTT TTGGAGATCT
1621CAACTCTGGC TGTGTACCGA TTGCCACACC CAGGCTGGAG GCCGGAGATG GTGGGCCTGC
1681TGTCGCTGTG GGACATGACC CGCATGCTGA ACATGCTCAT CGTGTTCCGC TTCCTGCGTA
1741TCATCCCCAG CATGAAGCCG ATGGCCGTGG TGGCCAGTAC CGTCCTGGGC CTGGTGCAGA
1801ACATGCGTGC TTTTGGCGGG ATCCTGGTGG TGGTCTACTA CGTATTTGCC ATCATTGGGA
1861TCAACTTGTT TAGAGGCGTC ATTGTGGCTC TTCCTGGAAA CAGCAGCCTG GCCCCTGCCA
1921ATGGCTCGGC GCCCTGTGGG AGCTTCGAGC AGCTGGAGTA CTGGGCCAAC AACTTCGATG
1981ACTTTGCGGC TGCCCTGGTC ACTCTGTGGA ACTTGATGGT GGTGAACAAC TGGCAGGTGT
2041TTCTGGATGC ATATCGGCGC TACTCAGGCC CGTGGTCCAA GATCTATTTT GTATTGTGGT
2101GGCTGGTGTC GTCTGTCATC TGGGTCAACC TGTTTCTGGC CCTGATTCTG GAGAACTTCC
2161TTCACAAGTG GGACCCCCGC AGCCACCTGC AGCCCCTTGC TGGGACCCCA GAGGCCACCT
2221ACCAGATGAC TGTGGAGCTC CTGTTCAGGG ATATTCTGGA GGAGCCCGAG GAGGATGAGC
2281TCACAGAGAG GCTGAGCCAG CACCCGCACC TGTGGCTGTG CAGGTTGCCA ACTTTCTTGT
2341ACAAAGTTGG cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)