Construct: ORF ccsbBroadEn_10084
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012034.1_s300c1, BRDN0000398113
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- THEMIS (387357)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 387357 | THEMIS | thymocyte selection associated | NM_001010923.2 | 99.9% | 99.8% | 1888A>G |
| 2 | human | 387357 | THEMIS | thymocyte selection associated | XM_024446433.1 | 98.5% | 98.4% | 251_277del;1915A>G |
| 3 | human | 387357 | THEMIS | thymocyte selection associated | NM_001164687.1 | 94.4% | 94.3% | 0_1ins105;1783A>G |
| 4 | human | 387357 | THEMIS | thymocyte selection associated | NM_001164685.1 | 94.2% | 94.1% | 1757_1873del;2005A>G |
| 5 | human | 387357 | THEMIS | thymocyte selection associated | XM_011535814.2 | 93.1% | 93% | 0_1ins105;146_172del;1810A>G |
| 6 | human | 387357 | THEMIS | thymocyte selection associated | XM_017010848.1 | 90.9% | 90.6% | (many diffs) |
| 7 | human | 387357 | THEMIS | thymocyte selection associated | NM_001318531.1 | 84.8% | 84.7% | 0_1ins291;1597A>G |
| 8 | human | 387357 | THEMIS | thymocyte selection associated | XM_011535816.1 | 84.8% | 84.7% | 0_1ins291;1597A>G |
| 9 | human | 387357 | THEMIS | thymocyte selection associated | XM_024446434.1 | 84.8% | 84.7% | 0_1ins291;1597A>G |
| 10 | human | 387357 | THEMIS | thymocyte selection associated | XM_017010849.1 | 76% | 75.8% | 250_251ins459;1429A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1992
- ORF length:
- 1923
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGCATTATCA CTGGAAGAAT TCGTCCACTC CCTTGACCTC AGGACCCTAC 121 CCAGGGTTCT AGAAATCCAG GCAGGCATCT ATCTTGAAGG CTCTATTTAT GAAATGTTTG 181 GAAATGAATG CTGTTTTTCA ACAGGAGAAG TGATTAAAAT TACTGGTCTC AAAGTTAAGA 241 AGATCATAGC TGAAATTTGT GAGCAGATTG AAGGTTGTGA GTCTCTACAG CCATTTGAAC 301 TGCCTATGAA TTTTCCAGGT CTTTTTAAGA TTGTGGCTGA TAAAACTCCA TACCTTACTA 361 TGGAAGAAAT CACAAGGACC ATTCATATTG GACCAAGTAG ACTAGGGCAT CCTTGCTTCT 421 ATCATCAGAA GGATATAAAA CTAGAGAACC TCATCATAAA GCAGGGTGAG CAAATCATGC 481 TCAACTCAGT TGAAGAGATT GATGGAGAAA TAATGGTGAG CTGTGCAGTA GCAAGGAATC 541 ATCAAACTCA CTCATTTAAT TTGCCTTTGT CACAAGAAGG AGAATTCTAC GAGTGTGAAG 601 ATGAACGTAT TTACACTCTA AAGGAGATTG TTGAATGGAA GATTCCTAAG AACAGAACAA 661 GAACTGTAAA CCTTACAGAT TTTTCAAATA AGTGGGACTC AACGAATCCA TTTCCTAAAG 721 ACTTTTATGG TACCCTGATT CTCAAGCCTG TTTATGAAAT TCAAGGTGTG ATGAAATTTC 781 GAAAAGATAT AATCCGCATC CTCCCCAGTC TAGATGTCGA AGTCAAAGAC ATCACTGATT 841 CTTACGATGC TAACTGGTTT CTTCAGCTGT TATCAACAGA AGATCTTTTT GAAATGACTA 901 GTAAAGAGTT CCCCATAGTG ACTGAAGTCA TAGAAGCACC TGAAGGAAAC CACCTGCCCC 961 AAAGCATTTT ACAGCCTGGG AAAACCATTG TGATCCACAA AAAGTACCAG GCATCAAGAA 1021 TCTTAGCTTC AGAAATTAGA AGCAATTTTC CTAAAAGACA CTTCTTGATC CCCACTAGCT 1081 ATAAAGGCAA GTTCAAGCGG CGACCGAGGG AGTTCCCAAC GGCCTATGAC CTAGAGATCG 1141 CTAAGAGTGA AAAGGAGCCT CTTCACGTGG TGGCCACCAA AGCGTTTCAT TCCCCTCATG 1201 ACAAGCTGTC ATCCGTATCT GTTGGGGACC AGTTTCTGGT GCATCAGTCA GAGACGACTG 1261 AAGTCCTCTG TGAGGGAATA AAAAAAGTGG TGAATGTTCT GGCCTGTGAA AAAATCCTCA 1321 AAAAGTCCTA TGAGGCTGCG CTGCTCCCTT TGTACATGGA AGGAGGTTTT GTAGAGGTGA 1381 TTCATGATAA GAAACAGTAC CCGATTTCTG AGCTCTGTAA ACAGTTCCGT TTGCCCTTCA 1441 ATGTGAAGGT GTCTGTCAGG GATCTTTCCA TTGAAGAGGA CGTGTTGGCT GCCACACCAG 1501 GACTGCAGTT GGAGGAGGAC ATTACAGACT CTTACCTACT CATAAGTGAC TTTGCCAACC 1561 CCACGGAGTG CTGGGAAATT CCTGTGGGCC GCTTGAATAT GACTGTTCAG TTAGTTAGTA 1621 ATTTCTCTAG GGATGCAGAA CCATTTCTAG TCAGGACTCT GGTAGAAGAG ATCACTGAAG 1681 AGCAATATTA CATGATGCGG AGATATGAAA GCTCAGCCTC ACATCCCCCA CCTCGCCCTC 1741 CGAAACACCC CTCAGTAGAG GAAACAAAGT TAACCCTGCT AACCTTAGCA GAAGAAAGGA 1801 CGGTAGACCT GCCCAAGTCT CCCAAGCGTC ATCACGTAGA CATAACCAAG AAACTTCACC 1861 CAAATCAAGC TGGCCTGGAT TCAAAAGTAC TGATTGGTAG TCAGAATGAT TTGGTGGATG 1921 AAGAGAAAGA AAGGAGCAAC CGTGGGGCCA CAGCAGTAGC AGAAACATTC AAAAATGAAA 1981 AACATCAAAA ATTGCCAACT TTCTTGTACA AAGTtggcat tataagaaag cattgcttat 2041 caatttgttg caacgaac