Construct: ORF ccsbBroadEn_10359
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001621.1_s300c1, BRDN0000397226
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ECEL1P2 (347694)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 347694 | ECEL1P2 | endothelin converting enzym... | NR_028501.1 | 9.8% | 1_125del;255_1309del | |
| 2 | human | 9427 | ECEL1 | endothelin converting enzym... | NM_001290787.2 | 4.9% | 4.6% | (many diffs) |
| 3 | human | 9427 | ECEL1 | endothelin converting enzym... | NM_004826.4 | 4.9% | 4.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 195
- ORF length:
- 129
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCG CGAGATCGAA CGACTGAACC CGCTGCTCAT GCTGGAGGTC ATCGAGGACT 121 GCGGGGGCTG GGACCTGCGC GGCGCGGCGG AGCGCCGGGG GGTTGCTGCG CGATGGGACC 181 TCAACTGGCT GCTGTACCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct 241 tatcaatttg ttgcaacgaa c