Construct: ORF ccsbBroadEn_10539
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014332.1_s300c1, BRDN0000388701
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL23AP7 (118433)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6147 | RPL23A | ribosomal protein L23a | NM_000984.6 | 64.4% | 58% | (many diffs) |
| 2 | human | 56969 | RPL23AP32 | ribosomal protein L23a pseu... | NR_002229.2 | 50.9% | (many diffs) | |
| 3 | human | 285830 | HLA-F-AS1 | HLA-F antisense RNA 1 | NR_026973.1 | 46.1% | (many diffs) | |
| 4 | human | 649946 | RPL23AP64 | ribosomal protein L23a pseu... | NR_003040.2 | 44% | (many diffs) | |
| 5 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_000029.3 | 29.2% | (many diffs) | |
| 6 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_024529.1 | 25.9% | (many diffs) | |
| 7 | human | 284942 | RPL23AP82 | ribosomal protein L23a pseu... | NR_026982.1 | 24.7% | (many diffs) | |
| 8 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_024528.1 | 24.2% | (many diffs) | |
| 9 | human | 284942 | RPL23AP82 | ribosomal protein L23a pseu... | NR_026981.1 | 23.5% | (many diffs) | |
| 10 | human | 285830 | HLA-F-AS1 | HLA-F antisense RNA 1 | NR_026972.1 | 16.9% | (many diffs) | |
| 11 | human | 388574 | RPL23AP87 | ribosomal protein L23a pseu... | NR_029406.1 | 15.8% | (many diffs) | |
| 12 | human | 644128 | RPL23AP53 | ribosomal protein L23a pseu... | NR_003572.2 | 5.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 429
- ORF length:
- 363
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGTC ACTCACCTTC AGGCGGCCCA AGACACTGCG ACTCCGGAGG CAGCCCAGAT 121 ATCCTCAGAA GAGCACCCCC AGGAGAAACA AGCTTGGCCA CTATGCTATC ATCAAGTTTC 181 CGCTGACCAC TGAGTCGGCC GTGAAGAAGA TAGAAGAAAA CAACACGCTT GTGTTCACTG 241 TGGATGTTAA AGCCAACAAG CACCAGATCA GACAGGCTGT GAAGAAGCTC TATGACAGTG 301 ATGTGGCCAA GGTCACCACC CTGATTTGTC CTGATAAAGA GAAGAAGGCA TATGTTCGAC 361 TTGCTCCTGA TTATGATGCT TTCAATGTTG TAACAAAATT GGGATCACCT AAACTGAGTC 421 CAGCTGGCTA CCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa 481 tttgttgcaa cgaac