Construct: ORF ccsbBroadEn_10860

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002752.1_s300c1, BRDN0000394434
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRIA3 (2892)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2892 GRIA3 glutamate ionotropic recept... NM_000828.4 91.4% 91% (many diffs)
2 human 2892 GRIA3 glutamate ionotropic recept... NM_007325.5 90.2% 89.9% (many diffs)
3 human 2892 GRIA3 glutamate ionotropic recept... NM_001256743.2 15.2% 10.9% (many diffs)
4 mouse 53623 Gria3 glutamate receptor, ionotro... NM_001290451.1 86% 89.9% (many diffs)
5 mouse 53623 Gria3 glutamate receptor, ionotro... XM_011251015.1 86% 89.8% (many diffs)
6 mouse 53623 Gria3 glutamate receptor, ionotro... XM_017318546.1 86% 89.8% (many diffs)
7 mouse 53623 Gria3 glutamate receptor, ionotro... NM_001281929.1 84.8% 88.9% (many diffs)
8 mouse 53623 Gria3 glutamate receptor, ionotro... NM_016886.4 84.8% 88.9% (many diffs)
9 mouse 53623 Gria3 glutamate receptor, ionotro... XM_006541520.3 84.8% 88.8% (many diffs)
10 mouse 53623 Gria3 glutamate receptor, ionotro... XM_017318545.1 84.8% 88.8% (many diffs)
11 mouse 53623 Gria3 glutamate receptor, ionotro... XM_017318547.1 80.7% 82.4% (many diffs)
12 mouse 53623 Gria3 glutamate receptor, ionotro... NR_104053.1 43.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2541
ORF length:
2472
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGCCAGGCAG AAGAAAATGG GGCAAAGCGT GCTCCGGGCG GTCTTCTTTT
121TAGTCCTGGG GCTTTTGGGT CATTCTCACG GAGGATTCCC CAACACCATC AGCATAGGTG
181GACTTTTCAT GAGAAACACA GTGCAGGAGC ACAGCGCTTT CCGCTTTGCC GTGCAGTTAT
241ACAACACCAA CCAGAACACC ACCGAGAAGC CCTTCCATTT GAATTACCAC GTAGATCACT
301TGGATTCCTC CAATAGTTTT TCCGTGACAA ATGCTTTCTG CTCCCAGTTC TCGAGAGGGG
361TGTATGCCAT CTTTGGATTC TATGACCAGA TGTCAATGAA CACCCTGACC TCCTTCTGTG
421GGGCCCTGCA CACATCCTTT GTTACGCCTA GCTTCCCCAC TGACGCAGAT GTGCAGTTTG
481TCATCCAGAT GCGCCCAGCC TTGAAGGGCG CTATTCTGAG TCTTCTGGGT CATTACAAGT
541GGGAGAAGTT TGTGTACCTC TATGACACAG AACGAGGATT TTCCATCCTC CAAGCGATTA
601TGGAAGCAGC AGTGCAAAAC AACTGGCAAG TAACAGCAAG GTCTGTGGGA AACATAAAGG
661ACGTCCAAGA ATTCAGGCGC ATCATTGAAG AAATGGACAG GAGGCAGGAA AAGCGATACT
721TGATTGACTG CGAAGTCGAA AGGATTAACA CAATTTTGGA ACAGGTTGTG ATCCTAGGGA
781AACACTCAAG AGGTTATCAC TACATGCTCG CTAACCTGGG TTTTACTGAT ATTTTACTGG
841AAAGAGTCAT GCATGGGGGA GCCAACATTA CAGGTTTCCA GATTGTCAAC AATGAAAACC
901CTATGGTTCA GCAGTTCATA CAGCGCTGGG TGAGGCTGGA TGAAAGGGAA TTCCCTGAAG
961CCAAGAATGC ACCACTAAAG TATACATCTG CATTGACACA CGACGCAATA CTGGTCATAG
1021CAGAAGCTTT CCGCTACCTG AGGAGGCAGC GAGTAGATGT GTCCCGGAGA GGAAGTGCTG
1081GAGACTGCTT AGCAAATCCT GCTGTGCCCT GGAGTCAAGG AATTGATATT GAGAGAGCTC
1141TGAAAATGGT GCAAGTACAA GGAATGACTG GAAATATTCA ATTTGACACT TATGGACGTA
1201GGACAAATTA TACCATCGAT GTGTATGAAA TGAAAGTCAG TGGCTCTCGA AAAGCTGGCT
1261ACTGGAACGA GTATGAAAGG TTTGTGCCTT TCTCAGATCA GCAAATCAGC AATGACAGTG
1321CATCCTCAGA GAATCGGACC ATAGTAGTGA CTACCATTCT GGAATCACCA TATGTAATGT
1381ACAAGAAGAA CCATGAGCAA CTGGAAGGAA ATGAACGATA TGAAGGCTAT TGTGTAGACC
1441TAGCCTATGA AATAGCCAAA CATGTAAGGA TCAAATACAA ATTGTCCATC GTTGGTGACG
1501GGAAATATGG TGCAAGGGAT CCAGAGACTA AAATATGGAA CGGCATGGTT GGGGAACTTG
1561TCTATGGGAG AGCTGATATA GCTGTTGCTC CACTCACTAT AACATTGGTC CGTGAAGAAG
1621TCATAGATTT TTCAAAGCCA TTCATGAGCC TGGGCATCTC CATCATGATA AAGAAGCCTC
1681AGAAATCAAA ACCAGGCGTA TTCTCATTTC TGGATCCCCT GGCTTATGAA ATCTGGATGT
1741GCATTGTCTT TGCTTACATT GGAGTCAGCG TAGTTCTTTT CCTAGTCAGC AGGTTCAGTC
1801CTTATGAATG GCACTTGGAA GACAACAATG AAGAACCTCG TGACCCACAA AGTCCTCCTG
1861ATCCTCCAAA TGAATTTGGA ATATTTAACA GTCTTTGGTT TTCCTTGGGT GCCTTTATGC
1921AGCAAGGATG TGATATTTCT CCAAGATCAC TCTCCGGGCG CATTGTTGGA GGGGTTTGGT
1981GGTTCTTCAC CCTGATCATA ATTTCTTCCT ATACTGCCAA TCTCGCTGCT TTCCTGACTG
2041TGGAGAGGAT GGTTTCTCCC ATAGAGAGTG CTGAAGACTT AGCTAAACAG ACTGAAATTG
2101CATATGGGAC CCTGGACTCC GGTTCAACAA AAGAATTTTT CAGAAGATCC AAAATTGCTG
2161TGTACGAGAA AATGTGGTCT TACATGAAAT CAGCGGAGCC ATCTGTGTTT ACCAAAACAA
2221CAGCAGACGG AGTGGCCCGA GTGCGAAAGT CCAAGGGAAA GTTCGCCTTC CTGCTGGAGT
2281CAACCATGAA TGAGTACATT GAGCAGAGAA AACCATGTGA TACGATGAAA GTTGGTGGAA
2341ATCTGGATTC CAAAGGCTAT GGTGTGGCAA CCCCTAAAGG CTCAGCATTA GGAAATGCTG
2401TTAACCTGGC AGTATTAAAA CTGAATGAGC AAGGCCTCTT GGACAAATTG AAAAACAAAT
2461GGTGGTACGA CAAAGGAGAG TGCGGCAGCG GGGGCGGTGA CTCCAAGAAC GCCTGTAAAC
2521CTTGCAGTAT TGAAACTCAG TTGCCAACTT TCTTGTACAA AGTtggcatt ataagaaagc
2581attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)