Construct: ORF ccsbBroadEn_10916
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001197.1_s300c1, BRDN0000390327
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGL (3535)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100423062 | IGLL5 | immunoglobulin lambda like ... | NM_001178126.2 | 69.7% | 55.1% | (many diffs) |
| 2 | human | 100423062 | IGLL5 | immunoglobulin lambda like ... | NM_001256296.2 | 56.2% | 48.9% | (many diffs) |
| 3 | human | 723778 | MIR650 | microRNA 650 | NR_030755.1 | 6.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 771
- ORF length:
- 705
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaCTTTTG TACAAAAAAG 61 TTGGCATGGC CTGGGCTCTG CTCCTCCTCA CCCTCCTCAC TCAGGGCACA GGGTCCTGGG 121 CCCAGTCTGC CCTGACTCAG CCTCCCTCCG CGTCCGGGTC TCCTGGACAG TCAGTCACCA 181 TCTCCTGCAC TGGAACCAGC AGTGACGTTG GTGGTTATAA CTATGTCTCC TGGTACCAAC 241 AGCACCCAGG CAAAGCCCCC AAACTCATGA TTTATGAGGT CAATAAGCGG CCCTCAGGGG 301 TCCCTGATCG CTTCTCTGGC TCCAAGTCTG GCAACACGGC CTCCCTGACC GTCTCTGGGC 361 TCCAGGCTGA GGATGAGGCT GATTATTACT GCAGCTCATA TGCAGGCAGC AACAATTATG 421 TCTTCGGAAC TGGGACCAAG GTCACCGTCC TAGGTCAGCC CAAGGCCAAC CCCACTGTCA 481 CTCTGTTCCC GCCCTCCTCT GAGGAGCTCC AAGCCAACAA GGCCACACTA GTGTGTCTGA 541 TCAGTGACTT CTACCCGGGA GCTGTGACAG TGGCCTGGAA GGCAGATGGC AGCCCCGTCA 601 AGGCGGGAGT GGAGACCACC AAACCCTCCA AACAGAGCAA CAACAAGTAC GCGGCCAGCA 661 GCTACCTGAG CCTGACGCCC GAGCAGTGGA AGTCCCACAG AAGCTACAGC TGCCAGGTCA 721 CGCATGAAGG GAGCACCGTG GAGAAGACAG TGGCCCCTAC AGAATGTTCA TACCCAACTT 781 TCTTGTACAA AGTtggcatt ataagaaagc attgcttatc aatttgttgc aacgaac