Construct: ORF ccsbBroadEn_10918

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014468.1_s300c1, BRDN0000386797
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IHH (3549)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3549 IHH Indian hedgehog signaling m... NM_002181.4 71.1% 71.2% 1_354del;600G>A;1128T>C
2 mouse 16147 Ihh Indian hedgehog NM_010544.3 62.9% 67.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
945
ORF length:
879
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA CCAGTGGCCC GGTGTGAAGC TGCGGGTGAC CGAGGGCTGG GACGAGGACG
121GCCACCACTC AGAGGAGTCC CTGCATTATG AGGGCCGCGC GGTGGACATC ACCACATCAG
181ACCGCGACCG CAATAAGTAT GGACTGCTGG CGCGCTTGGC AGTGGAGGCC GGCTTTGACT
241GGGTGTATTA CGAGTCAAAG GCCCACGTGC ATTGCTCCGT CAAGTCCGAG CACTCGGCCG
301CAGCCAAGAC AGGCGGCTGC TTCCCTGCCG GAGCCCAGGT ACGCCTGGAG AGTGGGGCGC
361GTGTGGCCTT GTCAGCCGTG AGGCCGGGAG ACCGTGTGCT GGCCATGGGG GAGGATGGGA
421GCCCCACCTT CAGCGATGTG CTCATTTTCC TGGACCGCGA GCCTCACAGG CTGAGAGCCT
481TCCAGGTCAT CGAGACTCAG GACCCCCCAC GCCGCCTGGC ACTCACACCC GCTCACCTGC
541TCTTTACGGC TGACAATCAC ACGGAGCCGG CAGCCCGCTT CCGGGCCACA TTTGCCAGCC
601ACGTGCAGCC TGGCCAGTAC GTGCTGGTGG CTGGGGTGCC AGGCCTGCAG CCTGCCCGCG
661TGGCAGCTGT CTCTACACAC GTGGCCCTCG GGGCCTACGC CCCGCTCACA AAGCATGGGA
721CACTGGTGGT GGAGGATGTG GTGGCATCCT GCTTCGCGGC CGTGGCTGAC CACCACCTGG
781CTCAGTTGGC CTTCTGGCCC CTGAGACTCT TTCACAGCTT GGCATGGGGC AGCTGGACCC
841CGGGGGAGGG TGTGCATTGG TACCCCCAGC TGCTCTACCG CCTGGGGCGT CTCCTGCTAG
901AAGAGGGCAG CTTCCACCCA CTGGGCATGT CCGGGGCAGG GAGCTGCCCA ACTTTCTTGT
961ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)