Construct: ORF ccsbBroadEn_11163
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008226.1_s300c1, BRDN0000388530
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK4 (6789)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6789 | STK4 | serine/threonine kinase 4 | NR_147975.2 | 12.5% | 1_57del;175_935del | |
| 2 | human | 6789 | STK4 | serine/threonine kinase 4 | XM_017028033.2 | 10% | 10% | 118_1164del |
| 3 | human | 6789 | STK4 | serine/threonine kinase 4 | XM_011529020.2 | 8.5% | 7.1% | (many diffs) |
| 4 | human | 6789 | STK4 | serine/threonine kinase 4 | NM_001352385.2 | 8.4% | 8.4% | 118_1386del |
| 5 | human | 6789 | STK4 | serine/threonine kinase 4 | NM_006282.5 | 8% | 8% | 118_1461del |
| 6 | human | 6789 | STK4 | serine/threonine kinase 4 | XM_005260531.3 | 7.2% | 6% | (many diffs) |
| 7 | human | 6789 | STK4 | serine/threonine kinase 4 | XM_005260530.2 | 6.8% | 5.7% | (many diffs) |
| 8 | human | 6789 | STK4 | serine/threonine kinase 4 | NR_147974.2 | 6.1% | 1_57del;174_1891delinsG | |
| 9 | mouse | 58231 | Stk4 | serine/threonine kinase 4 | XM_006499964.3 | 10.3% | 11.4% | (many diffs) |
| 10 | mouse | 58231 | Stk4 | serine/threonine kinase 4 | XM_006499963.3 | 8.1% | 9% | (many diffs) |
| 11 | mouse | 58231 | Stk4 | serine/threonine kinase 4 | NM_021420.3 | 7.2% | 8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 183
- ORF length:
- 117
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGA GACGGTACAG CTGAGGAACC CGCCGCGCCG GCAGCTGAAA AAGTTGGATG 121 AAGATAGTTT AACCAAACAA CCAGAAGAAG TATTTGATGT CTTAGAGAAA CTTGGAGAAG 181 GGTGCCCAAC TTTCTTGTAC AAAGTtggca ttataagaaa gcattgctta tcaatttgtt 241 gcaacgaac