Transcript: Human XM_017028033.2

PREDICTED: Homo sapiens serine/threonine kinase 4 (STK4), transcript variant X10, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
STK4 (6789)
Length:
1324
CDS:
58..1224

Additional Resources:

NCBI RefSeq record:
XM_017028033.2
NBCI Gene record:
STK4 (6789)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147987 TGGATCGTTATGGAGTACTG pXPR_003 TGG 311 27% 4 0.8139 STK4 STK4 76075
2 BRDN0001149002 AGCTTTGTATACGCTGCCAT pXPR_003 AGG 124 11% 3 -0.1650 STK4 STK4 76074
3 BRDN0001148451 TTTAGGATACCATGGCCAAG pXPR_003 CGG 537 46% 6 -0.2895 STK4 STK4 76076
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017028033.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196901 GTTCTGTATCTGATATCATTC pLKO.1 380 CDS 100% 10.800 15.120 N STK4 n/a
2 TRCN0000001624 GCCCTCATGTAGTCAAATATT pLKO.1 302 CDS 100% 15.000 12.000 N STK4 n/a
3 TRCN0000001625 GCCAAGCGGAATACAGTGATA pLKO.1 592 CDS 100% 4.950 3.960 N STK4 n/a
4 TRCN0000279861 GCCAAGCGGAATACAGTGATA pLKO_005 592 CDS 100% 4.950 3.960 N STK4 n/a
5 TRCN0000001623 CCGGCCAGATTGTTGCTATTA pLKO.1 212 CDS 100% 13.200 9.240 N STK4 n/a
6 TRCN0000279921 CCGGCCAGATTGTTGCTATTA pLKO_005 212 CDS 100% 13.200 9.240 N STK4 n/a
7 TRCN0000195133 CACAGACTTATGGATCGTTAT pLKO.1 342 CDS 100% 10.800 7.560 N STK4 n/a
8 TRCN0000279862 CACAGACTTATGGATCGTTAT pLKO_005 342 CDS 100% 10.800 7.560 N STK4 n/a
9 TRCN0000195454 CCAGAGCTATGGTCAGATAAC pLKO.1 796 CDS 100% 10.800 7.560 N STK4 n/a
10 TRCN0000279919 CCAGAGCTATGGTCAGATAAC pLKO_005 796 CDS 100% 10.800 7.560 N STK4 n/a
11 TRCN0000196832 GATTCAGGAAATTGGATACAA pLKO.1 642 CDS 100% 5.625 3.938 N STK4 n/a
12 TRCN0000195305 CATCCAATGAGGGCAATCTTC pLKO.1 739 CDS 100% 4.950 3.465 N STK4 n/a
13 TRCN0000195540 CATGACTGATGGAGCCAATAC pLKO.1 1095 CDS 100% 10.800 6.480 N STK4 n/a
14 TRCN0000279918 CATGACTGATGGAGCCAATAC pLKO_005 1095 CDS 100% 10.800 6.480 N STK4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017028033.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11163 pDONR223 100% 10% 10% None 118_1164del n/a
2 ccsbBroad304_11163 pLX_304 0% 10% 10% V5 118_1164del n/a
3 TRCN0000473017 AAGAACATCCCGGTTGACTCGATT pLX_317 100% 10% 10% V5 118_1164del n/a
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