Construct: ORF ccsbBroadEn_11250
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014940.1_s300c1, BRDN0000387268
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSCA (8000)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8000 | PSCA | prostate stem cell antigen | NM_005672.5 | 92.1% | 92.6% | 0_1ins27;285T>C;342C>A |
| 2 | human | 8000 | PSCA | prostate stem cell antigen | NR_033343.2 | 29.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 435
- ORF length:
- 369
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAA GGCTGTGCTG CTTGCCCTGT TGATGGCAGG CTTGGCCCTG CAGCCAGGCA 121 CTGCCCTGCT GTGCTACTCC TGCAAAGCCC AGGTGAGCAA CGAGGACTGC CTGCAGGTGG 181 AGAACTGCAC CCAGCTGGGG GAGCAGTGCT GGACCGCGCG CATCCGCGCA GTTGGCCTCC 241 TGACCGTCAT CAGCAAAGGC TGCAGCTTGA ACTGCGTGGA TGACTCACAG GACTACTACG 301 TGGGCAAGAA GAACATCACG TGCTGTGACA CCGACTTGTG CAACGCCAGC GGGGCCCATG 361 CCCTGCAGCC GGCTGCCGCC ATCCTTGCGC TGCTCCCTGC ACTCGGCCTG CTGCTCTGGG 421 GACCCGGCCA GCTATACCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct 481 tatcaatttg ttgcaacgaa c