Construct: ORF ccsbBroadEn_11287

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010579.1_s300c1, BRDN0000394525
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NPFF (8620)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8620 NPFF neuropeptide FF-amide pepti... NM_001320296.2 100% 100%
2 human 8620 NPFF neuropeptide FF-amide pepti... NM_003717.4 75.3% 65.3% (many diffs)
3 human 114108587 ATF7-NPFF ATF7-NPFF readthrough NR_159377.1 14.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
417
ORF length:
348
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGTTCCGCAG CCTCCTACCA CTTGCCCCTG GAAGCCAGTC CCTTCCCCTT
121GTGACTTACG TGTCCAGGGT ATTTGCCCAT CTTCCTTCCC TGATACCCCC TTGGCACAGG
181AGGAAGACAG CGAACCCCTC CCACCACAGG ATGCCCAGAC CTCTGGGTCA CTGTTGCACT
241ACCTGCTCCA GGCAATGGAG AGACCTGGCC GGAGCCAAGC CTTCCTGTTT CAGCCCCAGA
301GGTTTGGCAG AAATACCCAG GGATCCTGGA GGAATGAATG GCTGAGTCCC CGGGCTGGAG
361AGGGGCTGAA TTCCCAGTTC TGGAGCCTGG CTGCCCCTCA ACGCTTTGGG AAGAAGTTGC
421CAACTTTCTT GTACAAAGTt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
481aac

Download FASTA (ORF) (Full)