Construct: ORF ccsbBroadEn_11365

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013330.1_s300c1, BRDN0000389351
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SELENOF (9403)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9403 SELENOF selenoprotein F NM_004261.5 38.1% 0% 1_256del;313_362del
2 human 9403 SELENOF selenoprotein F NM_203341.2 26% 0% 1_256del;372_373ins73
3 human 9403 SELENOF selenoprotein F NR_144513.1 11.7% 1_317del;374_423del;557_1603del
4 human 9403 SELENOF selenoprotein F NR_144512.1 11.6% 1_333del;390_439del;573_1619del
5 mouse 93684 Selenof selenoprotein F NM_053102.2 35.3% 7.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
255
ORF length:
189
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCA GGAGCTATTC TTGAAGTTTG TGGATGAAAA TTGGGAAGGT TCCCTCAAGT
121CCAAGTATGT CCGTGGTTCA GACCCTGTAT TAAAGCTTTT GGACGACAAT GGGAACATTG
181CTGAAGAACT GAGCATTCTC AAATGGAACA CAGACAGTGT AGAAGAATTC CTGAGTGAAA
241AGTTGGAACG CATATACCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct
301tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)