Construct: ORF ccsbBroadEn_11630

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF014805.1_s300c1, BRDN0000385776
• DNA Barcode: None
• Epitope Tag: None
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: NLGN4Y (22829)

Vector Information:

• Vector Backbone: pDONR223
• Pol II Cassette 1: n/a
• Pol II Cassette 2: n/a
• Selection Marker: n/a
• Visible Reporter: n/a
• Epitope Tag: n/a

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	22829	NLGN4Y	neuroligin 4 Y-linked	NM_001164238.1	52.3%	52.3%	1_366del
2	human	22829	NLGN4Y	neuroligin 4 Y-linked	XM_017030041.1	52.3%	52.3%	1_366del
3	human	22829	NLGN4Y	neuroligin 4 Y-linked	NM_001365584.1	14.7%	13.1%	(many diffs)
4	human	22829	NLGN4Y	neuroligin 4 Y-linked	NM_001365586.1	14.7%	13.1%	(many diffs)
5	human	22829	NLGN4Y	neuroligin 4 Y-linked	NM_001365588.1	14.7%	13.1%	(many diffs)
6	human	22829	NLGN4Y	neuroligin 4 Y-linked	XM_006724874.2	14.7%	13.1%	(many diffs)
7	human	22829	NLGN4Y	neuroligin 4 Y-linked	XM_011531427.2	14.7%	13.1%	(many diffs)
8	human	22829	NLGN4Y	neuroligin 4 Y-linked	XM_011531428.1	14.7%	13.1%	(many diffs)
9	human	22829	NLGN4Y	neuroligin 4 Y-linked	XM_011531429.2	14.7%	13.1%	(many diffs)
10	human	22829	NLGN4Y	neuroligin 4 Y-linked	XM_011531430.2	14.7%	13.1%	(many diffs)
11	human	22829	NLGN4Y	neuroligin 4 Y-linked	XM_024452490.1	14.7%	13.1%	(many diffs)
12	human	57502	NLGN4X	neuroligin 4 X-linked	XM_005274564.3	13.9%	12.1%	(many diffs)
13	human	57502	NLGN4X	neuroligin 4 X-linked	XM_005274565.2	13.9%	12.1%	(many diffs)
14	human	57502	NLGN4X	neuroligin 4 X-linked	XM_005274566.4	13.9%	12.1%	(many diffs)
15	human	57502	NLGN4X	neuroligin 4 X-linked	XM_006724504.3	13.9%	12.1%	(many diffs)
16	human	57502	NLGN4X	neuroligin 4 X-linked	XM_011545547.2	13.9%	12.1%	(many diffs)
17	human	57502	NLGN4X	neuroligin 4 X-linked	XM_011545548.2	13.9%	12.1%	(many diffs)
18	human	22829	NLGN4Y	neuroligin 4 Y-linked	XM_017030039.1	13.2%	10.7%	(many diffs)
19	human	22829	NLGN4Y	neuroligin 4 Y-linked	NM_001365590.1	12.3%	10.8%	(many diffs)
20	human	22829	NLGN4Y	neuroligin 4 Y-linked	NM_001365591.1	12.3%	10.8%	(many diffs)
21	human	22829	NLGN4Y	neuroligin 4 Y-linked	NM_001365592.1	12.3%	10.8%	(many diffs)
22	human	22829	NLGN4Y	neuroligin 4 Y-linked	NM_001365593.1	12.3%	10.8%	(many diffs)
23	human	22829	NLGN4Y	neuroligin 4 Y-linked	NM_014893.4	12.3%	10.8%	(many diffs)
24	human	22829	NLGN4Y	neuroligin 4 Y-linked	XM_017030036.1	12.3%	10.8%	(many diffs)
25	human	57502	NLGN4X	neuroligin 4 X-linked	NM_001282145.2	11.8%	10.3%	(many diffs)
26	human	57502	NLGN4X	neuroligin 4 X-linked	NM_001282146.1	11.8%	10.3%	(many diffs)
27	human	57502	NLGN4X	neuroligin 4 X-linked	NM_020742.3	11.8%	10.3%	(many diffs)
28	human	57502	NLGN4X	neuroligin 4 X-linked	NM_181332.3	11.8%	10.3%	(many diffs)
29	human	57502	NLGN4X	neuroligin 4 X-linked	XM_017029691.1	11.8%	10.3%	(many diffs)
30	human	57502	NLGN4X	neuroligin 4 X-linked	XM_017029692.1	11.8%	10.3%	(many diffs)
31	human	57502	NLGN4X	neuroligin 4 X-linked	XM_017029693.1	11.8%	10.3%	(many diffs)
32	human	22829	NLGN4Y	neuroligin 4 Y-linked	NR_046355.1	6.8%		(many diffs)
33	human	22829	NLGN4Y	neuroligin 4 Y-linked	NR_028319.1	5.4%		(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

468

ORF length:

402

Sequence:

1	gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61	TTGGCATGCT GCCCATCTGG TTTACCACCA GTTTGGATAC TTTGATGACC TATGTTCAAG
121	ATCAAAATGA AGACTGCCTT TACTTAAACA TCTATGTGCC CATGGAAGAT GGAACCAACA
181	TAAAGAGAAA TGCAGACGAT ATAACCAGTA ATGACCATGG TGAAGATAAA GATATTCATG
241	AACAGAACAG TAAGAAGCCT GTTATGGTCT ATATCCATGG GGGATCTTAC ATGGAGGGAA
301	CCGGTAACAT GATTGATGGC AGCATTTTGG CCAGCTATGG GAACGTCATC GTTATCACCA
361	TTAACTACCG TCTGGGAATA CTAGGTATGC AAGAGGCACG TTTGTGTGGG AGCTCAAAAA
421	TGTTTAATTA TTTTAAATCT CCTTTCACTA ATTTAATAAA TTTTTTTTGC CCAACTTTCT
481	TGTACAAAGT tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac

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