Construct: ORF ccsbBroadEn_11962
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000352.1_s300c1, BRDN0000392485
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX47 (51202)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51202 | DDX47 | DEAD-box helicase 47 | NM_016355.4 | 70.9% | 70.9% | 1_396del |
| 2 | human | 51202 | DDX47 | DEAD-box helicase 47 | NM_201224.2 | 60.2% | 60.2% | 1_396del;748_749ins147 |
| 3 | mouse | 67755 | Ddx47 | DEAD (Asp-Glu-Ala-Asp) box ... | NM_026360.3 | 62.6% | 69.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1035
- ORF length:
- 969
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGTC TCAATCTTTG GCCCTTGCAA AAAAACCACA TATAATAATA GCAACTCCTG 121 GTCGACTGAT TGACCACTTG GAAAATACGA AAGGTTTCAA CTTGAGAGCT CTCAAATACT 181 TGGTCATGGA TGAAGCCGAC CGAATACTGA ATATGGATTT TGAGACAGAG GTTGACAAGA 241 TCCTCAAAGT GATTCCTCGA GATCGGAAAA CATTCCTCTT CTCTGCCACC ATGACCAAGA 301 AGGTTCAAAA ACTTCAGCGA GCAGCTCTGA AGAATCCTGT GAAATGTGCC GTTTCCTCTA 361 AATACCAGAC AGTTGAAAAA TTACAGCAAT ATTATATTTT TATTCCCTCT AAATTCAAGG 421 ATACCTACCT GGTTTATATT CTAAATGAAT TGGCTGGAAA CTCCTTTATG ATATTCTGCA 481 GCACCTGTAA TAATACCCAG AGAACAGCTT TGCTACTGCG AAATCTTGGC TTCACTGCCA 541 TCCCCCTCCA TGGACAAATG AGTCAGAGTA AGCGCCTAGG ATCCCTTAAT AAGTTTAAGG 601 CCAAGGCCCG TTCCATTCTT CTAGCAACTG ACGTTGCCAG CCGAGGTTTG GACATACCTC 661 ATGTAGATGT GGTTGTCAAC TTTGACATTC CTACCCATTC CAAGGATTAC ATCCATCGAG 721 TAGGTCGAAC AGCTAGAGCT GGGCGCTCCG GAAAGGCTAT TACTTTTGTC ACACAGTATG 781 ATGTGGAACT CTTCCAGCGC ATAGAACACT TAATTGGGAA GAAACTACCA GGTTTTCCAA 841 CACAGGATGA TGAGGTTATG ATGCTGACAG AACGCGTCGC TGAAGCCCAA AGGTTTGCCC 901 GAATGGAGTT AAGGGAGCAT GGAGAAAAGA AGAAACGCTC GCGAGAGGAT GCTGGAGATA 961 ATGATGACAC AGAGGGTGCT ATTGGTGTCA GGAACAAGGT GGCTGGAGGA AAAATGAAGA 1021 AGCGGAAAGG CCGTTACCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct 1081 tatcaatttg ttgcaacgaa c