Construct: ORF ccsbBroadEn_12062
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014137.1_s300c1, BRDN0000392064
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EGLN1 (54583)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54583 | EGLN1 | egl-9 family hypoxia induci... | NM_022051.2 | 30% | 30% | 1_894del |
| 2 | human | 54583 | EGLN1 | egl-9 family hypoxia induci... | XM_005273166.5 | 19.9% | 19.9% | 1_894del;1149_1150ins129 |
| 3 | mouse | 112405 | Egln1 | egl-9 family hypoxia-induci... | NM_053207.2 | 28.9% | 30.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 450
- ORF length:
- 384
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGT TGCTTGTTAT CCGGGCAATG GAACGGGTTA TGTACGTCAT GTTGATAATC 121 CAAATGGAGA TGGAAGATGT GTGACATGTA TATATTATCT TAATAAAGAC TGGGATGCCA 181 AGGTAAGTGG AGGTATACTT CGAATTTTTC CAGAAGGCAA AGCCCAGTTT GCTGACATTG 241 AACCCAAATT TGATAGACTG CTGTTTTTCT GGTCTGACCG TCGCAACCCT CATGAAGTAC 301 AACCAGCATA TGCTACAAGG TACGCAATAA CTGTTTGGTA TTTTGATGCA GATGAGAGAG 361 CACGAGCTAA AGTAAAATAT CTAACAGGTG AAAAAGGTGT GAGGGTTGAA CTCAATAAAC 421 CTTCAGATTC GGTCGGTAAA GACGTCTTCT ACCCAACTTT CTTGTACAAA GTtggcatta 481 taagaaagca ttgcttatca atttgttgca acgaac