Construct: ORF ccsbBroadEn_12134

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016084.1_s300c1, BRDN0000396356
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MCUB (55013)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55013 MCUB mitochondrial calcium unipo... XM_006714246.3 80.6% 80.7% 1_177del;411T>C
2 human 55013 MCUB mitochondrial calcium unipo... NM_017918.5 73.7% 73.8% 1_264del;498T>C
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
810
ORF length:
744
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTT GTCAACAGTT GGTTCATTCC TTCAGGACCT ACAAAATGAA GATAAGGGTA
121TCAAAACTGC AGCCATCTTC ACAGCAGATG GCAACATGAT TTCAGCTTCT ACCTTGATGG
181ATATTTTGCT AATGAATGAT TTTAAACTTG TCATTAATAA AATAGCATAT GATGTGCAGT
241GTCCAAAGAG AGAAAAACCA AGTAATGAGC ACACTGCTGA GATGGAACAC ATGAAATCCT
301TGGTTCACAG ACTATTTACA ATCTTGCATT TAGAAGAGTC TCAGAAAAAG AGAGAGCACC
361ATTTACTGGA GAAAATTGAC CACCTGAAGG AACAGCTGCA GCCCCTTGAA CAGGTGAAAG
421CTGGAATAGA AGCTCATTCG GAAGCCAAAA CCAGTGGACT CCTGTGGGCT GGATTGGCAC
481TGCTGTCCAT TCAGGGTGGG GCACTGGCCT GGCTCACGTG GTGGGTGTAC TCCTGGGATA
541TCATGGAGCC AGTTACATAC TTCATCACAT TTGCAAATTC TATGGTCTTT TTTGCATACT
601TTATAGTCAC TCGACAGGAT TATACTTACT CAGCTGTTAA GAGTAGGCAA TTTCTTCAGT
661TCTTCCACAA GAAATCAAAG CAACAGCACT TTGATGTGCA GCAATACAAC AAGTTAAAAG
721AAGACCTTGC TAAGGCTAAA GAATCCCTGA AACAGGCGCG TCATTCTCTC TGTTTGCAAA
781TGCAAGTAGA AGAACTCAAT GAAAAGAATT ACCCAACTTT CTTGTACAAA GTtggcatta
841taagaaagca ttgcttatca atttgttgca acgaac

Download FASTA (ORF) (Full)