Construct: ORF ccsbBroadEn_12343
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016288.1_s300c1, BRDN0000394057
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLK4 (57396)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57396 | CLK4 | CDC like kinase 4 | NM_020666.3 | 11.1% | 11% | 162_1443delinsG |
| 2 | mouse | 12750 | Clk4 | CDC like kinase 4 | NM_007714.6 | 9.9% | 10.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 229
- ORF length:
- 162
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG GTACAAAAAA 61 GTTGGCATGC GGCATTCCAA AAGAACTCAC TGTCCTGATT GGGATAGCAG AGAAAGCTGG 121 GGACATGAAA GCTATCGTGG AAGTCACAAG CGGAAGAGGA GATCTCATAG TAGCACACAA 181 GAGAACAGGC ATTGTAAACC ACATCACCAG TTTAAAGAAT CTGATTGGTA CCCAACTTTC 241 TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac