Transcript: Human NM_020666.3

Homo sapiens CDC like kinase 4 (CLK4), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
CLK4 (57396)
Length:
2504
CDS:
106..1551

Additional Resources:

NCBI RefSeq record:
NM_020666.3
NBCI Gene record:
CLK4 (57396)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146339 GCTATCGTGGAAGTCACAAG pXPR_003 CGG 81 6% 2 0.3383 CLK4 CLK4 77800
2 BRDN0001147054 CCTCACTCTAGATGAAATCG pXPR_003 TGG 481 33% 5 0.2239 CLK4 CLK4 77799
3 BRDN0001145430 AACGAGCTGCTTCACGGTAA pXPR_003 CGG 590 41% 6 -0.0202 CLK4 CLK4 77797
4 BRDN0001146113 ATGAGCGAGATTATCGGGAC pXPR_003 CGG 204 14% 3 -0.1067 CLK4 CLK4 77798
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_020666.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000256044 ATTGTTGAACGGGTGATTATT pLKO_005 1967 3UTR 100% 15.000 21.000 N CLK4 n/a
2 TRCN0000265730 CCTTGAATGAGCGAGATTATC pLKO_005 287 CDS 100% 13.200 18.480 N CLK4 n/a
3 TRCN0000001352 CTGGCAATGATGGAACGAATA pLKO.1 1261 CDS 100% 10.800 15.120 N CLK4 n/a
4 TRCN0000001353 CGATGTGTCCAGATGCTAGAA pLKO.1 766 CDS 100% 4.950 6.930 N CLK4 n/a
5 TRCN0000001350 GTGTCCAGTGATAAATGTGAT pLKO.1 1803 3UTR 100% 4.950 6.930 N CLK4 n/a
6 TRCN0000194961 CGTTCAGAAATCCAAGTATTA pLKO.1 709 CDS 100% 13.200 10.560 N CLK4 n/a
7 TRCN0000219100 AGACTGTGTCAGTCAACTAAA pLKO_005 1601 3UTR 100% 13.200 9.240 N Clk4 n/a
8 TRCN0000256045 AGGATATGTTCCTAGACATTA pLKO_005 351 CDS 100% 13.200 9.240 N CLK4 n/a
9 TRCN0000196441 GAATCAGTGGTCTTACTATAT pLKO.1 1557 3UTR 100% 13.200 9.240 N CLK4 n/a
10 TRCN0000001351 GCATCCTTTCTTTGACTTATT pLKO.1 1518 CDS 100% 13.200 9.240 N CLK4 n/a
11 TRCN0000265734 TCGTTCAGAAATCCAAGTATT pLKO_005 708 CDS 100% 13.200 9.240 N CLK4 n/a
12 TRCN0000361161 TTTGACCTGGTTCGAAGAATG pLKO_005 1450 CDS 100% 10.800 7.560 N Clk4 n/a
13 TRCN0000010605 TGACGAATACAGGAATGACTA pLKO.1 324 CDS 100% 4.950 3.465 N CLK4 n/a
14 TRCN0000197156 GCACACAAGAGAACAGGCATT pLKO.1 212 CDS 100% 4.050 2.835 N CLK4 n/a
15 TRCN0000265717 ACAGAGCTCCCGAGGTCATTT pLKO_005 1133 CDS 100% 13.200 7.920 N CLK4 n/a
16 TRCN0000218637 AGTCTGACTATGTAGTCAAAT pLKO_005 992 CDS 100% 1.320 0.792 N Clk4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_020666.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491429 CTCCCGTCGCCGTGCATTGGACCA pLX_317 26.6% 100% 100% V5 (not translated due to prior stop codon) n/a
2 TRCN0000489812 TTTTGATATCCTAGGTATCTTGGG pLX_317 28.3% 99.8% 99.7% V5 993T>C;1443_1444insG n/a
3 TRCN0000487864 TCCAGGACTTCTACTATTCCGACA pLX_317 22.2% 99.7% 99.7% V5 (not translated due to prior stop codon) 1443_1444insTTG n/a
4 ccsbBroadEn_12343 pDONR223 100% 11.1% 11% None 162_1443delinsG n/a
5 ccsbBroad304_12343 pLX_304 0% 11.1% 11% V5 162_1443delinsG n/a
6 TRCN0000473098 CATCTACGTTTCGCAGTCTTGCAG pLX_317 100% 11.1% 11% V5 162_1443delinsG n/a
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