Construct: ORF ccsbBroadEn_12467
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001540.1_s300c1, BRDN0000393972
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HS1BP3 (64342)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64342 | HS1BP3 | HCLS1 binding protein 3 | XM_017004701.1 | 65.8% | 66.4% | 546C>T;623_624insGTTGGT;628_939delinsTGTTGT |
| 2 | human | 64342 | HS1BP3 | HCLS1 binding protein 3 | XM_017004698.1 | 53.2% | 53.6% | 546C>T;623_624insGTTGGT;628_1164delinsTGTTGT |
| 3 | human | 64342 | HS1BP3 | HCLS1 binding protein 3 | NM_022460.4 | 52.6% | 53% | 546C>T;623_624insGTTGGT;628_1176delinsTGTTGT |
| 4 | human | 64342 | HS1BP3 | HCLS1 binding protein 3 | XM_017004697.2 | 52.2% | 52.6% | 546C>T;623_624insGTTGGT;628_1185delinsTGTTGT |
| 5 | human | 64342 | HS1BP3 | HCLS1 binding protein 3 | XM_017004696.2 | 52.1% | 52.5% | 546C>T;623_624insGTTGGT;628_1188delinsTGTTGT |
| 6 | human | 64342 | HS1BP3 | HCLS1 binding protein 3 | XM_017004700.1 | 44.3% | 44.6% | (many diffs) |
| 7 | human | 64342 | HS1BP3 | HCLS1 binding protein 3 | XM_017004699.2 | 43.9% | 44.1% | (many diffs) |
| 8 | mouse | 58240 | Hs1bp3 | HCLS1 binding protein 3 | NM_021429.3 | 43.8% | 42.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCA GTCCCCGGCG GTGCTCGTCA CCTCCAGGCG ACTTCAGAAT GCCCACACTG 121 GCCTCGACCT GACTGTGCCC CAGCACCAGG AGGTACGGGG CAAGATGATG TCTGGACACG 181 TGGAGTACCA GATCCTGGTG GTGACCCGTC TGGCTGCGTT CAAGTCGGCC AAGCACAGGC 241 CCGAGGATGT CGTCCAGTTC TTGGTCTCCA AAAAGTACAG CGAGATTGAG GAGTTTTACC 301 AGAAACTGAG CAGTCGTTAT GCAGCAGCCA GCCTCCCCCC ACTACCCAGG AAGGTCCTGT 361 TTGTTGGGGA GTCTGACATC CGGGAGAGGA GAGCCGTGTT CAATGAGATC CTGCGCTGTG 421 TCTCCAAGGA TGCCGAGTTG GCAGGCAGCC CAGAGCTGCT AGAGTTCTTA GGTACCAGAT 481 CCCCAGGGGC TGCAGGGCTC ACCAGCAGAG ATTCCTCTGT CCTGGATGGC ACAGACAGTC 541 AGACAGGGAA TGATGAAGAG GCTTTCGACT TTTTTGAGGA GCAAGACCAA GTGGCAGAAG 601 AGGGTCCGCC TGTCCAGAGC CTGAAGGGCG AGGATGCTGA GGAATCCTTG GAGGAGGAGG 661 AGGCGCTGGA CCCTCTGGGC ATTATGCGGT TGGTCTCCTG TTGTTGCCCA ACTTTCTTGT 721 ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c