Transcript: Human XM_017004700.1

PREDICTED: Homo sapiens HCLS1 binding protein 3 (HS1BP3), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
HS1BP3 (64342)
Length:
2490
CDS:
240..1319

Additional Resources:

NCBI RefSeq record:
XM_017004700.1
NBCI Gene record:
HS1BP3 (64342)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017004700.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000138003 GAGAGCCGTGTTCAATGAGAT pLKO.1 464 CDS 100% 4.950 3.960 N HS1BP3 n/a
2 TRCN0000280802 GAGAGCCGTGTTCAATGAGAT pLKO_005 464 CDS 100% 4.950 3.960 N HS1BP3 n/a
3 TRCN0000137034 CAGAAACTGAGCAGTCGTTAT pLKO.1 375 CDS 100% 10.800 7.560 N HS1BP3 n/a
4 TRCN0000137135 CAAGGAACTGTTCAGAGTTGA pLKO.1 1046 CDS 100% 4.950 3.465 N HS1BP3 n/a
5 TRCN0000280800 CAAGGAACTGTTCAGAGTTGA pLKO_005 1046 CDS 100% 4.950 3.465 N HS1BP3 n/a
6 TRCN0000138279 CGAGATGGACATCTTGCAGTA pLKO.1 1250 CDS 100% 4.050 2.835 N HS1BP3 n/a
7 TRCN0000297887 CGAGATGGACATCTTGCAGTA pLKO_005 1250 CDS 100% 4.050 2.835 N HS1BP3 n/a
8 TRCN0000137245 GTTCAGAGTTGAAGAGGACTT pLKO.1 1055 CDS 100% 4.050 2.835 N HS1BP3 n/a
9 TRCN0000137939 GAGCTGCTAGAGTTCTTAGGT pLKO.1 528 CDS 100% 3.000 2.100 N HS1BP3 n/a
10 TRCN0000280724 GAGCTGCTAGAGTTCTTAGGT pLKO_005 528 CDS 100% 3.000 2.100 N HS1BP3 n/a
11 TRCN0000137760 GAAGCCCAAGAAACATCCCAA pLKO.1 770 CDS 100% 2.640 1.848 N HS1BP3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017004700.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12466 pDONR223 100% 44.4% 44.6% None 0_1ins99;524_525insGTTGGT;529_1077delinsTGTTGT n/a
2 TRCN0000473173 ACGTGAATTTGGGACATGCTGCCT pLX_317 46.1% 44.4% 44.6% V5 0_1ins99;524_525insGTTGGT;529_1077delinsTGTTGT n/a
3 ccsbBroadEn_12467 pDONR223 100% 44.3% 44.6% None (many diffs) n/a
4 ccsbBroad304_12467 pLX_304 0% 44.3% 44.6% V5 (many diffs) n/a
5 TRCN0000474420 TTGCTGAGTACCGGGTCAGTGAAT pLX_317 82.2% 44.3% 44.6% V5 (many diffs) n/a
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