Construct: ORF ccsbBroadEn_12534

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015643.1_s300c1, BRDN0000385682
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DDX54 (79039)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79039 DDX54 DEAD-box helicase 54 NM_001111322.2 23.5% 21.5% (many diffs)
2 human 79039 DDX54 DEAD-box helicase 54 NM_024072.4 23.4% 21.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
738
ORF length:
672
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCA CCTCGGATGT GGAGCCGGAC ACCCGGGAGA TGGTGCGTGC CCAGAACAAG
121AAGAAGAAGA AGTCTGGAGG CTTCCAGTCC ATGGGCCTGA GCTACCCGGT GTTCAAAGGC
181ATCATGAAGA AGGGGGAGCC TTTGAGCAGC AGGCAGCTGG CGCTGTCCTG GACTTGATGG
241GGGATGAAGC CCAGAACCTG ACGAGGGGCC GGCAGCAGCT CAAGTGGGAC CGTAAGAAGA
301AGCGGTTTGT GGGACAGTCA GGACAGGAAG ACAAGAAGAA GATTAAGACA GAGAGCGGCC
361GCTACATCAG CAGCTCCTAC AAGCGAGACC TCTATCAGAA GTGGAAACAG AAACAGAAAA
421TTGATGATCG TGACTCGGAC GAAGAAGGGG CATCTGACCG GCGAGGCCCA GAGCGAAGAG
481GTGGGAAGCG AGACCGTGGC CAAGCAGGTG CATCCCGGCC CCACGCCCCA GGCACCCCTG
541CAGGCCGAGT CCGCCCGGAA CTCAAGACCA AGCAGCAGAT CCTGAAGCAG CGGCGCCGGG
601CCCAGAAGCT GCACTTCCTG CAGCGTGGTG GCCTCAAGCA GCTCTCTGCC CGCAACCGCC
661GCCGCGTCCA GGAGCTGCAG CAGGGCGCCT TCGGCCGGGG TGCCCGCTCC AAGAAGGGCA
721AGATGCGGAA GAGGATGTGC CCAACTTTCT TGTACAAAGT tggcattata agaaagcatt
781gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)