Construct: ORF ccsbBroadEn_12743
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001187.1_s300c1, BRDN0000392368
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKR1E2 (83592)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | NM_001040177.3 | 95.8% | 95.6% | 921_960delinsG |
| 2 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XM_024448225.1 | 91.2% | 90.8% | 837_838insGTGTTTGATTTTGAATT;840_841ins64 |
| 3 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XM_011519715.2 | 89.2% | 86.5% | (many diffs) |
| 4 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XM_017016743.1 | 87.7% | 82.7% | (many diffs) |
| 5 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XM_011519718.2 | 84.6% | 81.7% | (many diffs) |
| 6 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XM_024448224.1 | 83.6% | 83.4% | 204_205ins117;804_843delinsG |
| 7 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XM_011519720.2 | 80.9% | 72.2% | (many diffs) |
| 8 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XM_011519722.2 | 80.9% | 72.2% | (many diffs) |
| 9 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XM_017016744.1 | 80.9% | 72.2% | (many diffs) |
| 10 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XR_001747221.1 | 79.4% | 1_115del;952_1070del;1152_1153insATG | |
| 11 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | NM_001271021.1 | 78% | 77.8% | 579_580ins171;750_789delinsG |
| 12 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XM_011519719.2 | 76.1% | 66.8% | (many diffs) |
| 13 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XM_011519724.2 | 72.5% | 69.7% | (many diffs) |
| 14 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XM_011519725.2 | 68.1% | 65.5% | (many diffs) |
| 15 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | NM_001271025.1 | 65.2% | 65% | 457_458ins294;627_666delinsG |
| 16 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XR_930519.2 | 61.3% | (many diffs) | |
| 17 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XR_001747222.1 | 60.2% | (many diffs) | |
| 18 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XR_001747220.1 | 58.4% | (many diffs) | |
| 19 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XR_002957024.1 | 57.7% | (many diffs) | |
| 20 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XM_017016745.1 | 57.3% | 57% | 0_1ins312;525_526insGTGTTTGATTTTGAATT;528_529ins64 |
| 21 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | NR_073126.1 | 55.6% | (many diffs) | |
| 22 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XM_024448226.1 | 54.7% | 51.8% | (many diffs) |
| 23 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | NR_073125.1 | 53.3% | 1_148del;1070_1725del | |
| 24 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XR_930518.2 | 45.5% | (many diffs) | |
| 25 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | XR_930520.2 | 44.6% | (many diffs) | |
| 26 | human | 83592 | AKR1E2 | aldo-keto reductase family ... | NR_073127.1 | 43.4% | 1_148del;727_728ins171;899_1554del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 987
- ORF length:
- 921
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGG AGATATCCCA GCCGTGGGCC TCAGCTCCTG GAAGGCTTCT CCAGGGAAAG 121 TGACCGAGGC AGTGAAAGAG GCCATTGACG CAGGGTACCG GCACTTCGAC TGTGCTTACT 181 TTTACCACAA TGAGAGGGAG GTTGGAGCAG GGATCCGTTG CAAGATCAAG GAAGGCGCTG 241 TAAGACGGGA GGATCTGTTC ATTGCCACTA AGCTGTGGTG CACCTGCCAT AAGAAGTCCT 301 TGGTGGAAAC AGCATGCAGA AAGAGTCTCA AGGCCTTGAA GCTGAACTAT TTGGACCTCT 361 ACCTCATACA CTGGCCCATG GGTTTCAAGC CTCCTCATCC AGAATGGATC ATGAGCTGCA 421 GTGAACTTTC CTTCTGCCTC TCACATCCTC GAGTGCAGGA CTTGCCTCTG GACGAGAGCA 481 ACATGGTTAT TCCCAGTGAC ACGGACTTCC TGGACACGTG GGAGGCCATG GAGGACCTGG 541 TGATCACCGG GCTGGTGAAG AACATCGGGG TGTCAAACTT CAACCATGAA CAGCTTGAGA 601 GGCTTTTGAA TAAGCCTGGG TTGAGGTTCA AGCCACTAAC CAACCAGATT GAGTGCCACC 661 CATATCTTAC TCAGAAGAAT CTGATCAGTT TTTGCCAATC CAGAGATGTG TCCGTGACTG 721 CTTACCGTCC TCTTGGTGGC TCGTGTGAGG GGGTTGACCT GATAGACAAC CCTGTGATCA 781 AGAGGATTGC AAAGGAGCAC GGCAAGTCTC CTGCTCAGAT TTTGATCCGA TTTCAAATCC 841 AGAGGAATGT GATAGTGATC CCCGGATCTA TCACCCCAAG TCACATTAAA GAGAATATCC 901 AGGTGTTTGA TTTTGAATTA ACACAGCACG ATATGGATAA CATCCTCAGC CTAAACAGGA 961 ATCTCCGACT GGCCATGTTC CCCATGTACC CAACTTTCTT GTACAAAGTt ggcattataa 1021 gaaagcattg cttatcaatt tgttgcaacg aac