Construct: ORF ccsbBroadEn_13225
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007521.1_s300c1, BRDN0000391159
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BEST3 (144453)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144453 | BEST3 | bestrophin 3 | NM_001282615.2 | 100% | 100% | |
| 2 | human | 144453 | BEST3 | bestrophin 3 | NM_001282616.2 | 100% | 100% | |
| 3 | human | 144453 | BEST3 | bestrophin 3 | NM_001282614.1 | 17.1% | 13.8% | (many diffs) |
| 4 | human | 144453 | BEST3 | bestrophin 3 | NM_001282613.1 | 12.1% | 9.8% | (many diffs) |
| 5 | human | 144453 | BEST3 | bestrophin 3 | XM_011537961.1 | 12.1% | 9.8% | (many diffs) |
| 6 | human | 144453 | BEST3 | bestrophin 3 | NM_032735.3 | 10.2% | 8.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 282
- ORF length:
- 216
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttgNNATGTT CCTCATCTCT AGCAGTGTTC ACGGAAGCGA CGAGCACGGG CGCCTGCTTA 121 GAAGGACGCT GATGCGCTAC GTCAATCTCA CCTCCCTGCT CATCTTTCGC TCGGTGAGCA 181 CTGCTGTGTA CAAAAGATTT CCCACAATGG ACCACGTGGT TGAAGCAGAA AGAACTGGCA 241 TGAAACCCAT TCTGCCTTCA AGTTTTGAGA TGCAGAGCTT TTAGccaact ttcttgtaca 301 aagttggcat tataagaaag cattgcttat caatttgttg caacgaac