Construct: ORF ccsbBroadEn_13235

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007248.1_s300c1, BRDN0000457231
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
UNC45B (146862)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 146862 UNC45B unc-45 myosin chaperone B NM_001308281.1 99.9% 100% 381G>A
2 human 146862 UNC45B unc-45 myosin chaperone B XM_017024234.1 99.9% 100% 381G>A
3 human 146862 UNC45B unc-45 myosin chaperone B NM_001033576.2 91.4% 91.4% 381G>A;1452_1688del
4 human 146862 UNC45B unc-45 myosin chaperone B NM_001267052.2 91.4% 91.4% 381G>A;1452_1688del
5 human 146862 UNC45B unc-45 myosin chaperone B NM_173167.3 91.2% 91.2% 381G>A;1453_1695del
6 mouse 217012 Unc45b unc-45 myosin chaperone B NM_178680.4 81.2% 86.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2619
ORF length:
2550
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGCAGAGGTG GAAGCGGTAC AGCTGAAGGA GGAAGGAAAC CGGCATTTCC
121AGCTCCAGGA CTACAAGGCC GCCACAAATA GCTACAGCCA GGCCCTGAAG CTGACCAAGG
181ACAAGGCCCT GCTGGCCACG CTTTATCGGA ACCGGGCAGC CTGTGGCCTG AAAACGGAGA
241GCTACGTCCA GGCAGCTTCA GATGCCTCCA GAGCCATCGA CATCAACTCC TCGGACATCA
301AGGCTCTGTA TCGGCGATGC CAGGCACTGG AGCACCTGGG GAAGCTGGAC CAGGCCTTCA
361AAGACGTGCA GCGTTGTGCC ACCCTCGAGC CACGGAACCA GAACTTCCAG GAGATGCTGA
421GGAGACTCAA CACCAGCATT CAGGAGAAAC TCCGAGTGCA GTTCTCCACA GACTCGAGGG
481TACAGAAGAT GTTTGAGATC CTCTTGGATG AAAACAGTGA GGCTGATAAG CGGGAAAAGG
541CTGCCAACAA TCTCATTGTC CTAGGCCGTG AGGAAGCAGG GGCTGAGAAG ATCTTCCAGA
601ACAATGGAGT AGCCTTGCTA CTGCAGCTTC TGGACACTAA GAAGCCTGAG CTGGTGCTGG
661CTGCAGTGCG GACCCTGTCG GGCATGTGCA GCGGCCACCA AGCCAGAGCC ACAGTGATTC
721TGCATGCAGT GCGGATAGAC CGAATCTGTA GCCTCATGGC CGTGGAGAAT GAGGAGATGT
781CTCTGGCTGT CTGCAACCTG CTCCAAGCCA TCATTGACTC CTTGTCTGGG GAGGACAAGC
841GGGAGCATCG AGGGAAGGAG GAGGCCCTGG TTCTAGACAC CAAGAAGGAC CTGAAGCAGA
901TCACCAGCCA CCTGCTGGAC ATGCTAGTCA GCAAGAAGGT GTCTGGCCAG GGCAGGGATC
961AGGCGCTGAA CCTGCTCAAT AAGAATGTTC CCAGGAAGGA CCTTGCCATT CATGACAACT
1021CACGTACCAT CTATGTGGTG GATAATGGTC TGAGGAAGAT CCTGAAGGTT GTGGGGCAGG
1081TTCCAGATCT GCCATCCTGC CTGCCCCTGA CTGACAACAC CCGCATGCTG GCCTCTATCC
1141TCATCAACAA GCTCTATGAT GACCTGCGCT GTGACCCGGA GCGCGATCAC TTCCGCAAGA
1201TCTGTGAGGA ATATATCACG GGCAAGTTTG ACCCCCAGGA CATGGACAAG AACTTGAATG
1261CCATCCAGAC AGTGTCAGGG ATCCTGCAGG GCCCCTTTGA CCTGGGCAAC CAGCTGCTGG
1321GACTGAAAGG TGTGATGGAG ATGATGGTGG CACTATGTGG CTCAGAGCGC GAGACGGACC
1381AGCTGGTGGC CGTGGAGGCC CTCATCCATG CCTCCACGAA GCTCAGCCGC GCCACCTTCA
1441TCATCACCAA TGGAGTGTCA CTGCTCAAAC AGATCTACAA GACCACCAAA AATGAGAAGA
1501TCAAGATCCG CACACTGGTG ACCAGTGACA AGACCATCCT GTACTCGGTG GCCACCACCC
1561TGGTGAACTG CACCAACAGC TACGATGTCA AGGAGGTCAT CCCAGAGCTT GTCCAGCTCG
1621CCAAGTTCTC CAAGCAGCAT GTGCCCGAGG AACACCCCAA GGACAAGAAG GACTTTATAG
1681ACATGCGGGT GAAGCGGCTT CTGAAGGCGG GTGTCATCTC TGCCCTGGCT TGCATGGTGA
1741AAGCAGATAG TGCCATCCTC ACTGACCAGA CCAAGGAGCT GCTGGCCAGG GTATTCCTGG
1801CACTGTGTGA CAACCCAAAG GACCGAGGCA CCATTGTGGC TCAAGGTGGT GGCAAGGCCC
1861TGATTCCCCT GGCTTTGGAG GGCACAGATG TGGGCAAGGT GAAGGCAGCC CACGCTCTAG
1921CAAAGATCGC TGCTGTCTCC AATCCGGACA TTGCTTTTCC TGGGGAGCGG GTGTATGAGG
1981TGGTGCGGCC CCTTGTAAGA CTCTTGGACA CACAGAGGGA TGGGCTTCAG AACTATGAGG
2041CTCTCCTAGG CCTCACCAAC CTGTCTGGGC GGAGTGACAA ACTCCGGCAG AAGATCTTTA
2101AGGAGAGGGC CTTGCCAGAC ATCGAGAACT ACATGTTTGA GAATCATGAT CAGCTGCGGC
2161AGGCGGCCAC CGAGTGCATG TGCAACATGG TGCTCCACAA GGAGGTACAG GAAAGGTTCT
2221TGGCTGACGG GAATGACCGG CTGAAGCTGG TGGTGCTGCT CTGCGGGGAG GATGATGATA
2281AGGTGCAGAA TGCGGCTGCA GGGGCTCTGG CCATGCTGAC AGCAGCACAC AAGAAACTGT
2341GCCTCAAGAT GACTCAAGTG ACAACCCAGT GGTTGGAGAT CCTCCAGCGG CTTTGCCTGC
2401ACGACCAGCT GTCTGTCCAA CACCGGGGCC TGGTCATTGC CTACAACCTA CTGGCAGCCG
2461ATGCTGAGCT GGCCAAGAAG CTGGTGGAGA GTGAGCTGCT GGAGATCCTG ACTGTGGTGG
2521GCAAACAGGA GCCAGATGAG AAGAAGGCAG AAGTGGTTCA GACAGCCCGA GAATGTCTCA
2581TCAAGTGCAT GGATTATGGT TTCATTAAAC CAGTGTCTTT GCCAACTTTC TTGTACAAAG
2641TTGGcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

Download FASTA (ORF) (Full)