Construct: ORF ccsbBroadEn_13622

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009867.1_s300c1, BRDN0000387996
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
WDR86 (349136)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 349136 WDR86 WD repeat domain 86 NM_001284262.1 100% 100%
2 human 349136 WDR86 WD repeat domain 86 XM_011516148.1 70% 40.4% 341_404del;809_1062del
3 human 349136 WDR86 WD repeat domain 86 XM_011516150.1 70% 40.4% 341_404del;809_1062del
4 human 349136 WDR86 WD repeat domain 86 NM_198285.3 65.9% 65.9% 1_384del
5 human 349136 WDR86 WD repeat domain 86 XM_011516146.3 63.9% 63.1% (many diffs)
6 human 349136 WDR86 WD repeat domain 86 NM_001284261.2 62.8% 44.9% 342_343ins136;609_831del
7 human 349136 WDR86 WD repeat domain 86 XM_011516145.3 52.7% 33.7% (many diffs)
8 human 349136 WDR86 WD repeat domain 86 XM_011516144.3 52.5% 31.1% (many diffs)
9 human 349136 WDR86 WD repeat domain 86 XM_005249990.5 51.7% 51.5% (many diffs)
10 human 349136 WDR86 WD repeat domain 86 NM_001284260.2 51.4% 30.3% 1_384del;725_788del;1193_1446del
11 human 349136 WDR86 WD repeat domain 86 XM_011516147.3 48.5% 35.8% (many diffs)
12 human 349136 WDR86 WD repeat domain 86 XM_006715966.3 47.8% 44.3% (many diffs)
13 human 349136 WDR86 WD repeat domain 86 XM_005249989.4 45% 32% 1_384del;726_727ins136;993_1215del
14 human 349136 WDR86 WD repeat domain 86 XM_011516151.3 44% 36.2% (many diffs)
15 human 349136 WDR86 WD repeat domain 86 XM_011516152.2 40.1% 34.9% 1_384del;725_788del;927_927delGins266
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
813
ORF length:
744
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GTCCCGGGAG TTCCGGGGCC ACCGCAACTG CGTGCTGACC CTAGCCTACT
121CTGCCCCGTG GGACCTCCCC AGCACTCCCT GCGCGGAGGA GGCCGCGGCC GGGGGGCTTC
181TGGTGACCGG CAGCACAGAT GGCACAGCCA AGGTGTGGCA GGTGGCCAGC GGCTGCTGCC
241ACCAGACGCT GCGGGGCCAC ACGGGTGCAG TGCTGTGCCT AGTGCTAGAC ACGCCCGGCC
301ACACGGCCTT CACAGGCAGC ACCGACGCCA CCATCCGTGC CTGGGACATC CTGAGTGGGG
361AGCAGCTGCG GGTGTTCCGG GAGCACCGGG GCTCCGTCAT CTGTCTGGAG CTGGTGAACC
421GACTCGTGTA CTCTGGCAGC GCGGACAGGA CCGTCAAGTG CTGGCTGGCA GACACAGGGG
481AGTGTGTGCG CACGTTCACG GCCCACAGAC GCAACGTGAG CGCCCTCAAG TACCACGCGG
541GCACCTTGTT CACGGGCAGC GGGGACGCTT GCGCCCGGGC CTTCGACGCG CAGTCTGGAG
601AGCTGCGGAG GGTGTTCCGG GGCCACACAT TCATCATCAA CTGCATCCAG GTGCACGGCC
661AGGTGCTCTA CACCGCCTCG CACGACGGCG CCCTGCGCCT CTGGGACGTG CGCGGGCTCC
721GAGGTGCCCC GCGGCCCCCT CCGCCCATGC GCAGCCTCTC GCGGCTCTTC AGCAACAAGG
781TGGGCTGCGC CGCCGCGCCC CTGCAGCCGG CCTTGCCAAC TTTCTTGTAC AAAGTtggca
841ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)