Construct: ORF ccsbBroadEn_13722
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017238.1_s300c1, BRDN0000397788
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS14P3 (644068)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6208 | RPS14 | ribosomal protein S14 | NM_001025070.2 | 84.5% | 76.1% | (many diffs) |
| 2 | human | 6208 | RPS14 | ribosomal protein S14 | NM_001025071.2 | 84.5% | 76.1% | (many diffs) |
| 3 | human | 6208 | RPS14 | ribosomal protein S14 | NM_005617.4 | 84.5% | 76.1% | (many diffs) |
| 4 | human | 644068 | RPS14P3 | ribosomal protein S14 pseud... | NR_077246.1 | 59.7% | 1_307del;818_853del | |
| 5 | mouse | 102634709 | LOC102634709 | 40S ribosomal protein S14 | XR_001781586.1 | 79.6% | (many diffs) | |
| 6 | mouse | 20044 | Rps14 | ribosomal protein S14 | NM_020600.4 | 78.8% | 76.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 576
- ORF length:
- 510
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC ACCTGGAAAG GGGAAGGAAA AGAAGGAAGA ACAGGTCATC AACCTCGGAC 121 CTCAGGTGGC TGAAGGGGAG AATGTATTTG GTGTCTGCCA TATCTTTGCA TCCTTCAATG 181 ACACTTTTGT CCATGTCACG GATCTTTCTG GCAAACAAAC CATCTGCCGT GTGACTGGTG 241 GGATGAAGGT GAAGGCAGAC CGAGATGAAT CCTCGCCATA TGCTGCTATG TTGACCACCC 301 AGGATGTGGC CCAGAGGTGC AAGGAGCTGG GTATCATTGC CCTACACATC CAACTCCGGG 361 CCACAGGAGG AAATAGGACC AAGACCCTTG GACCTGGGGC CCAGTCGGCC CTCAGAGCCC 421 TTGCCTGCTC GGGTATGAAG ATCGGGAGGA TTGAGGACGT CACCCCCATC CCCTCTGACA 481 GCACTCTCAG GAAGGGGGTC ACCGTGGTCG CCGTCTGTGA ACAAGATTCC TCAAAATATT 541 TTCTGTTAAT AAATTGCCTT CATGTAAAAA ATAAATACCC AACTTTCTTG TACAAAGTtg 601 gcattataag aaagcattgc ttatcaattt gttgcaacga ac