Construct: ORF ccsbBroadEn_13832
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011756.1_s300c1, BRDN0000388747
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AP1S1 (1174)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1174 | AP1S1 | adaptor related protein com... | NM_001283.5 | 78.1% | 79.7% | 14T>G;382_383insCTTTCCCCTTTTCCC;384_474delinsC |
| 2 | mouse | 11769 | Ap1s1 | adaptor protein complex AP-... | NM_007457.2 | 74.1% | 79.7% | (many diffs) |
| 3 | mouse | 11769 | Ap1s1 | adaptor protein complex AP-... | XM_006504348.3 | 66.2% | 71% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 465
- ORF length:
- 399
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TAGAAAAAAG 61 TTGGCATGAT GCGGTTCAGG CTATTATTCA GCCGGCAGGG AAAACTGCGG CTGCAAAAAT 121 GGTACCTGGC CACTTCGGAC AAGGAACGGA AGAAGATGGT GCGCGAGCTC ATGCAGGTTG 181 TCCTGGCTCG AAAGCCCAAG ATGTGCAGCT TCCTGGAGTG GAGGGACCTC AAAGTTGTCT 241 ATAAGAGATA TGCCAGCCTC TACTTCTGCT GCGCCATCGA GGGCCAAGAC AATGAGCTCA 301 TCACACTGGA GCTGATCCAC CGATACGTGG AGCTCTTAGA CAAATACTTT GGCAGTGTGT 361 GCGAGCTGGA CATCATCTTC AACTTTGAGA AGGCCTACTT CATCCTGGAT GAGTTTTTGA 421 TGGGGGGGGA TGTCCAGGAC ACCTCCACTT TCCCCTTTTC CCACTGCCCA ACTTTCTTGT 481 ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c