Construct: ORF ccsbBroadEn_13910

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017602.1_s300c1, BRDN0000391834
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NDUFA5 (4698)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4698 NDUFA5 NADH:ubiquinone oxidoreduct... NM_005000.5 99.7% 99.1% 345delA
2 human 4698 NDUFA5 NADH:ubiquinone oxidoreduct... NM_001282421.2 95.9% 93.1% (many diffs)
3 human 4698 NDUFA5 NADH:ubiquinone oxidoreduct... NM_001282420.2 95.7% 93.9% (many diffs)
4 human 4698 NDUFA5 NADH:ubiquinone oxidoreduct... NM_001282419.2 86.7% 86.2% 19_20ins45;300delA
5 human 4698 NDUFA5 NADH:ubiquinone oxidoreduct... NM_001282422.2 66% 65.5% 65_66ins117;228delA
6 human 4698 NDUFA5 NADH:ubiquinone oxidoreduct... NM_001291304.1 56.6% 51.8% (many diffs)
7 human 4698 NDUFA5 NADH:ubiquinone oxidoreduct... XM_024446771.1 50.5% 50% 0_1ins171;174delA
8 human 4698 NDUFA5 NADH:ubiquinone oxidoreduct... NR_111925.1 6.1% (many diffs)
9 human 4698 NDUFA5 NADH:ubiquinone oxidoreduct... NR_104169.2 6% (many diffs)
10 human 4698 NDUFA5 NADH:ubiquinone oxidoreduct... NR_104168.2 5.9% (many diffs)
11 human 4698 NDUFA5 NADH:ubiquinone oxidoreduct... NR_111926.1 5.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
414
ORF length:
348
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GGGTGTGCTG AAGAAGACCA CTGGCCTTGT GGGATTGGCT GTGTGCAATA
121CTCCTCACGA GAGGCTAAGA ATATTGTACA CAAAGATTCT TGATGTTCTT GAGGAAATCC
181CTAAAAATGC AGCATATAGA AAGTATACAG AACAGATTAC AAATGAGAAG CTGGCTATGG
241TTAAAGCGGA ACCAGATGTT AAAAAATTAG AAGACCAACT TCAAGGCGGT CAATTAGAAG
301AGGTGATTCT TCAGGCTGAA CATGAACTAA ATCTGGCAAG AAAAATGAGG GAATGGAAAC
361TATGGGAGCC ATTAGTGGAA GAGCCTCCTG CCGATCAGTG GAAATGGCCA TATACCCAAC
421TTTCTTGTAC AAAGTtggca ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)