Construct: ORF ccsbBroadEn_14048
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004450.1_s300c1, BRDN0000456087
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA4D (10507)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10507 | SEMA4D | semaphorin 4D | NM_001142287.2 | 16% | 14.9% | (many diffs) |
| 2 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371198.1 | 16% | 14.9% | (many diffs) |
| 3 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371199.1 | 16% | 14.9% | (many diffs) |
| 4 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371200.1 | 16% | 14.9% | (many diffs) |
| 5 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371201.1 | 16% | 14.9% | (many diffs) |
| 6 | human | 10507 | SEMA4D | semaphorin 4D | XR_001746156.1 | 11.1% | (many diffs) | |
| 7 | human | 10507 | SEMA4D | semaphorin 4D | XR_001746153.1 | 11.1% | (many diffs) | |
| 8 | human | 10507 | SEMA4D | semaphorin 4D | NR_163886.1 | 8.8% | (many diffs) | |
| 9 | human | 10507 | SEMA4D | semaphorin 4D | NR_163887.1 | 8.7% | (many diffs) | |
| 10 | human | 10507 | SEMA4D | semaphorin 4D | NR_163884.1 | 8.7% | (many diffs) | |
| 11 | human | 10507 | SEMA4D | semaphorin 4D | NR_163885.1 | 8.6% | (many diffs) | |
| 12 | human | 10507 | SEMA4D | semaphorin 4D | NR_163888.1 | 8.5% | (many diffs) | |
| 13 | human | 10507 | SEMA4D | semaphorin 4D | NR_163889.1 | 8.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 68
- ORF end:
- 437
- ORF length:
- 369
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAGT 61 TGGCACCATG TGGACTCAGA GGTGGAAACG GTCTGTGTCC CCAGGTCCTG CAGACATTCG 121 CTTTGTCTGG GAGAAGAATG GGCGAGCTCT GGAGACCTGT GTCCCTGTGC AGACCCATGC 181 ACTGCCCGAT GGCAGGGCCC ATGCACTCAG CTGGCTGCAG GACGCCATCA GGGAAAGCGC 241 TGAGTATCGC TGCTCTGTCC TCTCCTCAGC AGGGAACAAG ACTTCGAAGG TGCAGGTTGC 301 TGTGATGAGA CCTGAAGTGA CCCACCAGGA GAGGTGGACC AGAGAGCTCT CTGCCTGGAG 361 GGCTGTGGCT GGGNAGCACG ACCGGATGAT GCAGAGCTGG AGGAAGGCGT GGGAAAGCTG 421 TAGCAAGGAC ACCCTGTTGC CAACTTTCTT GTACAAAGTT GGcattataa gaaagcattg 481 cttatcaatt tgttgcaacg aac