Construct: ORF ccsbBroadEn_14533

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002551.2_s300c1, BRDN0000458525
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRK3 (157)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 157 GRK3 G protein-coupled receptor ... NM_005160.4 56.2% 56.2% 1161_2064delinsA
2 human 157 GRK3 G protein-coupled receptor ... NM_001362778.2 39.7% 39.8% 0_1ins339;822_1725delinsA
3 human 157 GRK3 G protein-coupled receptor ... XM_011529975.2 32.2% 32.2% 0_1ins495;666_1569delinsA
4 human 157 GRK3 G protein-coupled receptor ... XR_937823.1 12.7% 1_13del;1175_9074del
5 human 157 GRK3 G protein-coupled receptor ... XR_001755175.1 12.7% 1_13del;1174_9100delinsA
6 mouse 320129 Grk3 G protein-coupled receptor ... NM_177078.4 47.3% 52.9% (many diffs)
7 mouse 320129 Grk3 G protein-coupled receptor ... NM_001285806.1 33.9% 38.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1227
ORF length:
1161
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatggc ggacctggag gctgtgctgg ccgatgtcag ttacctgatg gccatggaga
121agagcaaggc gaccccggcc gcccgcgcca gcaagaggat cgtcctgccg gagcccagta
181tccggagtgt gatgcagaag taccttgcag agagaaatga aataaccttt gacaagattt
241tcaatcagaa aattggtttc ttgctattta aagatttttg tttgaatgaa attaatgaag
301ctgtacctca ggtgaagttt tatgaagaga taaaggaata tgaaaaactt gataatgagg
361aagaccgcct ttgcagaagt cgacaaattt atgatgccta catcatgaag gaacttcttt
421cctgttcaca tcctttctca aagcaagctg tagaacacgt acaaagtcat ttatccaaga
481aacaagtgac atcaactctt tttcagccat acatagaaga aatttgtgaa agccttcgag
541gtgacatttt tcaaaaattt atggaaagtg acaagttcac tagattttgt cagtggaaaa
601acgttgaatt aaatatccat ttgaccatga atgagttcag tgtgcatagg attattggac
661gaggaggatt cggggaagtt tatggttgca ggaaagcaga cactggaaaa atgtatgcaa
721tgaaatgctt agataagaag aggatcaaaa tgaaacaagg agaaacatta gccttaaatg
781aaagaatcat gttgtctctt gtcagcacag gagactgtcc tttcattgta tgtatgacct
841atgccttcca taccccagat aaactctgct tcatcctgga tctgatgaac gggggcgatt
901tgcactacca cctttcacaa cacggtgtgt tctctgagaa ggagatgcgg ttttatgcca
961ctgaaatcat tctgggtctg gaacacatgc acaatcggtt tgttgtctac agagatttga
1021agccagcaaa tattctcttg gatgaacatg gacacgcaag aatatcagat cttggtcttg
1081cctgcgattt ttccaaaaag aagcctcatg cgagtgttgg cacccatggg tacatggctc
1141ccgaggtgct gcagaagggg acggcctatg acagcagtgc cgactggttc tccctgggct
1201gcatgctttt caaacttctg agaggatgcc caactttctt gtacaaagtt ggcattataa
1261gaaagcattg cttatcaatt tgttgcaacg aac

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