Construct: ORF ccsbBroadEn_14582
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018963.1_s300c1, BRDN0000458259
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CHEK1 (1111)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001114121.2 | 98.6% | 45.4% | (many diffs) |
| 2 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001114122.2 | 98.6% | 45.4% | (many diffs) |
| 3 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001274.5 | 98.6% | 45.4% | (many diffs) |
| 4 | human | 1111 | CHEK1 | checkpoint kinase 1 | XM_017017146.2 | 98.6% | 45.4% | (many diffs) |
| 5 | human | 1111 | CHEK1 | checkpoint kinase 1 | XM_024448337.1 | 98.6% | 45.4% | (many diffs) |
| 6 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001244846.1 | 91.4% | 48.8% | (many diffs) |
| 7 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001330428.1 | 78.8% | 25.4% | (many diffs) |
| 8 | human | 1111 | CHEK1 | checkpoint kinase 1 | NM_001330427.1 | 69.7% | 25.2% | (many diffs) |
| 9 | human | 1111 | CHEK1 | checkpoint kinase 1 | XM_011542560.2 | 69.7% | 25.2% | (many diffs) |
| 10 | human | 1111 | CHEK1 | checkpoint kinase 1 | NR_045205.1 | 38% | (many diffs) | |
| 11 | human | 1111 | CHEK1 | checkpoint kinase 1 | NR_045204.1 | 28.4% | (many diffs) | |
| 12 | mouse | 12649 | Chek1 | checkpoint kinase 1 | NM_007691.5 | 88.1% | 43.9% | (many diffs) |
| 13 | mouse | 12649 | Chek1 | checkpoint kinase 1 | XM_017313117.1 | 69% | 23.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 95
- ORF end:
- 827
- ORF length:
- 732
- Sequence:
-
1 AATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCATGGCA GTGCCCTTTG TGGAAGACTG 121 GGACTTGGTG CAAACCCTGG GAGAAGGTGC CTATGGAGAA GTTCAACTTG CTGTGAATAG 181 AGTAACTGAA GAAGCAGTCG CAGTGAAGAT TGTAGATATG AAGCGTGCCG TAGACTGTCC 241 AGAAAATATT AAGAAAGAGA TCTGTATCAA TAAAATGCTA AATCATGAAA ATGTAGTAAA 301 ATTCTATGGT CACAGGAGAG AAGACAATAT CCAATATTTA TTTCTGGAGT ACTGTAGTGG 361 AGGAGAGCTT TTTGACAGAA TAGAGCCAGA CATAGGCATG CCTGAACCAG ATGCTCAGAG 421 ATTCTTCCAT CAACTCATGG CAGGGGTGGT TTATCTGCAT GGTATTGGAA TAACTCACAG 481 GGATATTAAA CCAGAAAATC TTCTGTTGGA TGAAAGGGAT AACCTCAAAA TCTCAGACTT 541 TGGCTTGGCA ACAGTATTTC GGTATAATAA TCGTGAGCGT TTGTTGAACA AGATGTGTGG 601 TACTTTACCA TATGTTGCTC CAGAACTTCT GAAGAGAAGA GAATTTCATG CAGAACCAGT 661 TGATGTTTGG TCCTGTGGAA TAGTACTTAC TGCAATGCTC GCTGGAGAAT TGCCATGGGA 721 CNANCCCAGT GACCAGCTGT CAGGAGTATT TCTGACTGGA AAGAAAAAAA AACCANTNCC 781 TCNACCCTTG GAAAAAAATC GATTCTGCTC CTCTAGCTCT GCTGCATAAA ATCTTAGTTG 841 AGANNNCATC AGCANNNNTT ACCATTCCAG ACATCAAAAA AGATAGATGG TACAACAAAC 901 CCCCTCAAGA AAGGGGCNAA AAGGCCCCGA GTCACTTCAG GTGGTGTGTC AGAGTCTCCC 961 AGTGGATTTT CTAAGCACAT TCAATCCAAT TTGGACTTCT CTCCAGTAAA CAGTGCTTCT 1021 AGTGAAGAAA ATGTGAAGTA CTCCAGTTCT CAGCCAGAAC CCCGCACAGG TCTTTCCTTA 1081 TGGGATACCA GCCCCTCATA CATTGATAAA TTGGTACAAG GGATCAGCTT TTCCCAGCCC 1141 ACATGTCCTG ATCATATGCT TTTGAATAGT CAGTTACTTG GCACCCCAGG ATCCTCACAG 1201 AACCCCTGGC AGCGGTTGGT CAAAAGAATG ACACGATTCT TTACCAAATT GGATGCAGAC 1261 AAATCTTATC AATGCCTGAA AGAGACTTGT GAGAAGTTGG GCTATCAATG GAAGAAAAGT 1321 TGTATGAATC AGGTTACTAT ATCAACAACT GATAGGAGAA ACAATAAACT CATTTTCAAA 1381 GTGAATTTGT TAGAAATGGA TGATAAAATA TTGGTTGACT TCCGGCTTTC TAAGGGTGAT 1441 GGATTGGAGT TCAAGAGACA CTTCCTGAAG ATTAAAGGGA AGCTGATTGA TATTGTGAGC 1501 AGCCAGAAGG TTTGGCTTCC TGCCACATGC CCAACTTTCT TGTACAAAGT TGGCATTATA 1561 AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC 1621 ATTATTGCCA TCCAAGCTGA TTTCCCCCC