Construct: ORF ccsbBroadEn_14595
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018790.1_s300c1, BRDN0000458086
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CRKL (1399)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1399 | CRKL | CRK like proto-oncogene, ad... | NM_005207.4 | 99.5% | 22.8% | (many diffs) |
| 2 | human | 1399 | CRKL | CRK like proto-oncogene, ad... | NR_156180.2 | 26.6% | (many diffs) | |
| 3 | mouse | 12929 | Crkl | v-crk avian sarcoma virus C... | NM_007764.5 | 90.1% | 22.5% | (many diffs) |
| 4 | mouse | 12929 | Crkl | v-crk avian sarcoma virus C... | NM_001277231.1 | 30.7% | 32.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 549
- ORF length:
- 450
- Sequence:
-
1 AATGGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT 61 TTTTTATAAT GCCAACTTTG TACAAAAAAG TTGGCACCAT GTCCTCCGCC AGGTTCGACT 121 CCTCGGACCG CTCCGCCTGG TATATGGGGC CGGTGTCTCG CCAGGAGGCG CAGACCCGGC 181 TCCAGGGCCA GCGCCACGGT ATGTTCCTCG TCCGCGATTC TTCCACCTGC CCTGGGGACT 241 ATGTGCTGTC GGTGGTCCGA GAACTCGCGG GTCTTCCCAC TACATCATCA ACTCGCTGCC 301 CAACCGCCGT TTTAAGATCG GGGACCAGGA ATTTGACCAT TTGCCGGNCC TGCTGGAGTT 361 TTACAAGATC CACTACCTGG ACACCACCAC CCTCATCGAG CCTGCGCCCA GGTATCCAAG 421 CCCACCAATG GGATCTGTCT CAGCACCCAA CCTGCCTACA GCAGAAGATA ACCTGGAATA 481 TGTACGGACT CTGTATGATT TTCCTGGGAA TGATGCCGAA GACCTGCCCT TTAAAAAGGG 541 TGAGATCCTA GTGATAATAG AGAAGCCTGA AGAACAGTGG TGGAGTGCCC GGAACAAGGA 601 TGGCCGGGTT GGGATGATTC CTGTCCCTTA TGTCGAAAAG CTTGTGAGAT CCTCACCACA 661 CGGAAAGCAT GGAAATAGGA ATTCCAACAG TTATGGGATC CCAGAACCTG CTCATGCATA 721 CGCTCAACCT CAGACCACAA CTCCTCTACC TGCAGTTTCC GGTTCTCCTG GGGCAGCAAT 781 CACCCCTTTG CCATCCACAC AGAATGGACC TGTCTTTGCG AAAGCAATCC AGAAAAGAGT 841 ACCCTGTGCT TATGACAAGA CTGCCTTGGC ATTAGAGGTT GGTGACATCG TGNAAGTCAC 901 AAGGATGAAT ATAAATGGCC AGTGGGAAGG CGAAGTGAAC GGGCGCAAAG GGCTTTTCCC 961 CTTTACGCAC GTCAAAATCT TTGACCCTCA AAACCCAGAT GAAAACGAGT TGCCAACTTT 1021 CTTGTACAAA GTTGGCATTA TAAGAAAGCA TTGCTTATCA ATTTGTTGCA ACGAACAGGT 1081 CACTATCAGT CAAAATAAAA TCATTATTGC CATCCAGCTG ATTC