Construct: ORF ccsbBroadEn_14608
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018964.1_s300c1, BRDN0000458260
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DGKG (1608)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1608 | DGKG | diacylglycerol kinase gamma | NM_001346.3 | 99% | 27.8% | (many diffs) |
| 2 | human | 1608 | DGKG | diacylglycerol kinase gamma | NM_001080744.2 | 95.9% | 28.7% | (many diffs) |
| 3 | human | 1608 | DGKG | diacylglycerol kinase gamma | NM_001080745.2 | 94.1% | 29.2% | (many diffs) |
| 4 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | NM_138650.2 | 87% | 22.3% | (many diffs) |
| 5 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | XM_017316850.1 | 82.2% | 21.1% | (many diffs) |
| 6 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | NM_001346673.1 | 82.2% | 23.5% | (many diffs) |
| 7 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | XM_017316851.1 | 77.6% | 22.1% | (many diffs) |
| 8 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | XM_017316852.1 | 72.6% | 23.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 95
- ORF end:
- 767
- ORF length:
- 672
- Sequence:
-
1 AATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCATGGGT GAAGAACGGT GGGTCTCCCT 121 CACTCCAGAA GAATTTGACC AACTCCAGAA ATATTCAGAA TATTCCTCCA AGAAGATAAA 181 AGATGCCTTG ACTGAATTTA ATGAGGGTGG GAGCCTCAAA CAATATGACC CACATGAGCC 241 GATTAGCTAT GATGTCTTCA AGCTGTTCAT GAGGGCGTAC CTGGAGGTGG ACCTTCCCCA 301 GCCACTGAGC ACTCACCTCT TCCTGGCCTT CAGCCAGAAG CCCAGACACG AGACCTCTGA 361 CCACCCGACG GAGGGAGCCA GCAACAGTGA GGCCAACAGC GCAGATACTA ATATACAGAA 421 TGCAGATAAT GCCACCAAAG CAGACGAGGC CTGTGCCCCT GATACTGAAT CAAATATGGC 481 TGAGAAGCAA GCACCAGCTG AAGACCAAGT GGCTGCGAGC CCCCTGGAAC CCCCCGTCCC 541 TCGGTCTTCA AGCTCGGAAT CCCCAGTGGT GTACCTGAAG GATGTTGTGT GCTACCTGTC 601 CCTGCTGGAG ACGGGGAGGC CTCAGGATAA GCTGGAGTTC ATGTTTCGCC TCTATGATTC 661 AGATGAGAAC GGTCTCCTGG ACCAAGCGGA GATGGATTGC ATTGTCAACC AAATGCTGCA 721 TATTGCCCAG TACCTGGAGT GGGATCCCAC AGAGCTGAGC CTATATTGAA GGAGATGCTG 781 CAAGGGATGG ACTACGACCG GGACGGCTTT GTGTCTCTAC AGGAATGGGT CCATGGAGGA 841 TGACACATCC CATGCTGTCC TCCTGGGGAT GATGACTCTG GCTCCATGGG GATGGGCGGC 901 ACGCCTGGAA CATGaagcac ttcaagaaac caacctactg caacttctgc catatcatgc 961 tcatgggcgt ccgcaagcaa ggcctgtgct gcacttactg taaatacact gtccacgaac 1021 gctgtgtgtc caaaaacatt cctggttgtg tcaaaacgta ctcaaaagcc aaaaggagtg 1081 gtgaggtgat gcagcacgca tgggtggaag ggaactcctc cgtcaagtgt gaccggtgcc 1141 acaaaagtat caagtgctac cagagtgtca ccgcgcggca ctgcgtgtgg tgccggatga 1201 cgtttcaccg caaatgtgaa ttatcaacgt tgtgtgacgg tggggaactc agagaccaca 1261 tcttactgcc cacctccata tgccccatca cccgggacag gccaggtgag aagtctgatg 1321 gctgcgtgtc cgccaagggc gaacttgtca tgcagtataa gatcatcccc accccgggta 1381 cccaccccct gctggtcttg gtgaacccca agagtggagg gagacaagga gaaagaattc 1441 ttcggaaatt ccactatctg ctcaacccca aacaagtttt caacctggac aatggggggc 1501 ctactccagg gttgaacttt ttccgtgata ctccagactt ccgtgttttg gcctgtggtg 1561 gagatgggac agttggctgg attttggatt gcattgataa ggccaacttt gcaaagcatc 1621 caccagtggc tgtcctgcct cttggaacag gaaatgacct TGCCCGTTGT TTCCGCGGGG 1681 AAGGAGGTTA TGAAGGGGGG CAGCTTGACA AAAATCCTGA AAGACATTGA GCAGAGCCCC 1741 TTGGTGATGC TGGACCGCTG CATCTGGNAA GTCATCCCCA GAGAGGAAGT GAAAACGGGG 1801 ACCCAGGTCC CATACAGCAT CATGAACAAC TATTTCTCCA TTGGTGTGGA CGCTTCCATT 1861 GCACACAGAT TCCATGTGAT GAGAGAGAAA CATCCTGAAA AATTCAACAG CAGGATGAAG 1921 AACAAGCTGT GGTACTTTGA ATTTGGCACC TCGGAGACTT TTGCAGCGAC CTGCAAGAAA 1981 CTCCACGACC ACATTGAGTT GGAGTGTGAT GGGGTTGGGG TGGACCTGAG CAACATCTTC 2041 CTGGAAGGCA TTGCCATTCT CAACATTCCC AGCATGTACG GAGGCACCAA TCTCTGGGGA 2101 GAAAACAAGA AGAACCGGGC TGTGATCCGG GAAAGCAGGA AGGGTGTCAC TGACCCCAAA 2161 GAACTGAAAT TCTGCGTTCA AGACCTCAGT GACCAGCTCC TTGAAGTGGT GGGGCTAGAA 2221 GGAGCCATGG AGATGGGGCA GATCTACACC GGCCTGAAGA GTGCAGGCAG GAGGCTGGCC 2281 CAGTGCGCCT CTGTCACCAT CAGGACAAAC AAGCTGCTGC CAATGCAAGT GGATGGAGAA 2341 CCCTGGATGC AGCCATGTTG CACGATTAAA ATTACTCACA AGAACCAAGC GCCCATGATG 2401 ATGGGGCCTC CCCAGAAGAG CAGCTTCTTC TCGTTGAGAA GGAAGAGCCG TTCAAAAGAC 2461 TTGCCAACTT TCTTGTACAA AGTTGGCATT ATAAGAAAGC ATTGCTTATC AATTTGTTGC 2521 AACGAACAGG TCACTATCAG TCAAAATAAA ATCATTATTG CCATCCAGCC TGAATCCCCC 2581 C