Construct: ORF ccsbBroadEn_14624
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018840.1_s300c1, BRDN0000458136
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MARK2 (2011)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2011 | MARK2 | microtubule affinity regula... | NM_004954.5 | 99.6% | 99.4% | (many diffs) |
| 2 | human | 2011 | MARK2 | microtubule affinity regula... | NM_001163296.2 | 98.2% | 97.6% | (many diffs) |
| 3 | human | 2011 | MARK2 | microtubule affinity regula... | NM_001163297.2 | 97.6% | 97.3% | (many diffs) |
| 4 | human | 2011 | MARK2 | microtubule affinity regula... | XM_011544796.1 | 96% | 95.1% | (many diffs) |
| 5 | human | 2011 | MARK2 | microtubule affinity regula... | XM_011544795.1 | 95.8% | 95% | (many diffs) |
| 6 | human | 2011 | MARK2 | microtubule affinity regula... | XM_011544794.1 | 94.8% | 94% | (many diffs) |
| 7 | human | 2011 | MARK2 | microtubule affinity regula... | XM_017017311.1 | 94.7% | 93.5% | (many diffs) |
| 8 | human | 2011 | MARK2 | microtubule affinity regula... | XM_011544797.1 | 94.6% | 93.4% | (many diffs) |
| 9 | human | 2011 | MARK2 | microtubule affinity regula... | XM_011544798.1 | 94% | 93.1% | (many diffs) |
| 10 | human | 2011 | MARK2 | microtubule affinity regula... | NM_001039469.2 | 91.5% | 91.3% | (many diffs) |
| 11 | human | 2011 | MARK2 | microtubule affinity regula... | XM_017017310.1 | 90.8% | 90.6% | (many diffs) |
| 12 | human | 2011 | MARK2 | microtubule affinity regula... | XM_011544791.1 | 89.5% | 88.7% | (many diffs) |
| 13 | human | 2011 | MARK2 | microtubule affinity regula... | XM_011544790.1 | 89.4% | 88.6% | (many diffs) |
| 14 | human | 2011 | MARK2 | microtubule affinity regula... | NM_017490.4 | 88.5% | 88.3% | (many diffs) |
| 15 | human | 2011 | MARK2 | microtubule affinity regula... | XM_011544789.1 | 88.4% | 87.6% | (many diffs) |
| 16 | human | 2011 | MARK2 | microtubule affinity regula... | XM_011544792.1 | 88.2% | 87.1% | (many diffs) |
| 17 | human | 2011 | MARK2 | microtubule affinity regula... | XM_011544793.1 | 87.5% | 86.7% | (many diffs) |
| 18 | human | 2011 | MARK2 | microtubule affinity regula... | XR_949801.2 | 80.3% | (many diffs) | |
| 19 | human | 2011 | MARK2 | microtubule affinity regula... | XR_949802.2 | 71.3% | (many diffs) | |
| 20 | human | 2011 | MARK2 | microtubule affinity regula... | XR_002957128.1 | 46.8% | (many diffs) | |
| 21 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | NM_001080388.2 | 92.2% | 97.3% | (many diffs) |
| 22 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | NM_001080389.2 | 91.1% | 96.1% | (many diffs) |
| 23 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | XM_006526652.3 | 88.8% | 92.3% | (many diffs) |
| 24 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | XM_006526654.3 | 88.6% | 91.8% | (many diffs) |
| 25 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | XM_017318058.1 | 87.9% | 91.4% | (many diffs) |
| 26 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | XM_017318059.1 | 87.8% | 92.8% | (many diffs) |
| 27 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | NM_007928.3 | 85.8% | 90.6% | (many diffs) |
| 28 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | XM_006526648.3 | 82.8% | 86.1% | (many diffs) |
| 29 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | XM_006526647.2 | 82.6% | 85.9% | (many diffs) |
| 30 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | XM_006526650.3 | 82.6% | 85.5% | (many diffs) |
| 31 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | NM_001080390.2 | 81.7% | 86.3% | (many diffs) |
| 32 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | XM_011247139.1 | 79.7% | 84.4% | (many diffs) |
| 33 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | XM_006526643.2 | 79.2% | 83.6% | (many diffs) |
| 34 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | XM_006526642.3 | 78.2% | 81.3% | (many diffs) |
| 35 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | XM_006526641.3 | 77.4% | 80.4% | (many diffs) |
| 36 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | XM_006526644.3 | 75.4% | 79.7% | (many diffs) |
| 37 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | XM_006526645.2 | 75.4% | 79.7% | (many diffs) |
| 38 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | XM_006526646.3 | 75.4% | 79.7% | (many diffs) |
| 39 | mouse | 13728 | Mark2 | MAP/microtubule affinity re... | XM_006526656.3 | 72% | 72.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 97
- ORF end:
- 2269
- ORF length:
- 2172
- Sequence:
-
1 ACGGATTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT 61 TTTATAATGC CAACTTTGTA CAAAAAAGTT GGCACCATGT CCAGCGCTCG GACCCCCCTA 121 CCCACGCTGA ACGAGAGGGA CACGGAGCAG CCCACCTTGG GACACCTTGA CTCCAAGCCC 181 AGCAGTAAGT CCAACATGAT TCGGGGCCGC AACTCAGCCA CCTCTGCTGA TGAGCAGCCC 241 CACATTGGAA ACTACCGGCT CCTCAAGACC ATTGGCAAGG GTAATTTTGC CAAGGTGAAG 301 TTGGCCCGAC ACATCCTGAC TGGGAAAGAG GTAGCTGTGA AGATCATTGA CAAGACTCAA 361 CTGAACTCCT CCAGCCTCCA GAAACTATTC CGCGAAGTAA GAATAATGAA GGTTTTGAAT 421 CATCCCAACA TAGTTAAATT ATTTGAAGTG ATTGAGACTG AGAAAACGCT CTACCTTGTC 481 ATGGAGTACG CTAGTGGCGG AGAGGTATTT GATTACCTAG TGGCTCATGG CAGGATGAAA 541 GAAAAAGAGG CTCGAGCCAA ATTCCGCCAG ATAGTGTCTG CTGTGCAGTA CTGTCACCAG 601 AAGTTTATTG TCCATAGAGA CTTAAAGGCA GAAAACCTGC TCTTGGATGC TGATATGAAC 661 ATCAAGATTG CAGACTTTGG CTTCAGCAAT GAATTCACCT TTGGGAACAA GCTGGACACC 721 TTCTGTGGCA GTCCCCCTTA TGCTGCCCCA GAACTCTTCC AGGGCAAAAA ATATGATGGA 781 CCCGAGGTGG ATGTGTGGAG CCTAGGAGTT ATCCTCTATA CACTGGTCAG CGGATCCCTG 841 CCTTTTGATG GACAGAACCT CAAGGAGCTG CGGGAACGGG TACTTGAGGG AAAATACCGT 901 ATTCCATtct acatgtccac ggactgtgaa aacctgctta agaaatttct cattcttaat 961 cccagcaaga gaggcacttt agagcaaatc atgaaagatc gatggatgaa tgtgggtcac 1021 gaagatgatg aactaaagcc ttacgtggag ccactccctg actacaagga cccccggcgg 1081 acagagctga tggtgtccat gggttataca cgggaagaga tccaggactc gctggtgggc 1141 cagagataca acgaggtgat ggccacctat ctgctcctgg gctacaagag ctccgagctg 1201 gaaggcgaca ccatcaccct gaaaccccgg ccttcagctg atctgaccaa tagcagcgcc 1261 ccatccccat cccacaaggt acagcgcagc gtgtcggcca atcccaagca gcggcgcttc 1321 agcgaccagg ctggtcctgc cattcccacc tctaattctt actctaagaa gactcagagt 1381 aacaacgcag aaaataagcg gcctgaggag gaccgggagt cagggcggaa agccagcagc 1441 acagccaagg tgcctgccag ccccctgccc ggtctggaga ggaagCCCAC CCCCCAAACC 1501 CCCTCCACGA ACAGCGTCCT CTCCACCAGC ACAAATCGAA GCAGGAATTC CCCACTTTTG 1561 GAGCGGGCCA GCCTCGGCCA GGCCTCCATC CAGAATGGCA AAGACAGCAC AGCCCCCCAG 1621 CGTGTCCCTG TTGCCTCCCC ATCCGCCCAC AACATCAGCA GCAGTGGTGG AGCCCCAGAC 1681 CGAACTAACT TCCCCCGGGG TGTGTCCAGC CGAAGCACCT TCCATGCTGG GCAGCTCCGA 1741 CAGGTGCGGG ACCAGCAGAA TTTGCCCTAC GGTGTGACCC CAGCCTCTCC CTCTGGCCAC 1801 AGCCAGGGCC GGCGGGGGGC CTCTGGGAGC ATCTTCAGCA AGTTCACCTC CAAGTTTGTA 1861 CGCAGGAACC TGAATGAACC TGAAAGCAAA GACCGAGTGG AGACGCTCAG ACCTCACGTG 1921 GTGGGCAGTG GCGGCAACGA CAAAGAAAAG GAAGAATTTC GGGAGGCCAA GCCCCGCTCC 1981 CTCCGCTTCA CGTGGAGTAT GAAGACCACG AGCTCCATGG AGCCCAACGA GATGATGCGG 2041 GAGATCCGCA AGGTGCTGGA CGCGAACAGC TGCCAGAGCG AGCTGCATGA GAAGTACATG 2101 CTGCTGTGCA TGCACGGCAC GCCGGGCCAC GAGGACTTCG TGCAGTGGGA GATGGAGGTG 2161 TGCAAACTGC CGCGGCTCTC TCTCAACGGG GTTCGATTTA AGCGGATATC GGGCACCTCC 2221 ATGGCCTTCA AAAACATTGC CTCCAAAATA GCCAACGAGC TGAAGCTTTT GCCAACTTTC 2281 TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC 2341 ACTATCAGTC AAAATAAAAT CATTATTGCC ATCCAGCTGA TCCAG