Construct: ORF ccsbBroadEn_14634
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018927.1_s300c1, BRDN0000458223
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ERN1 (2081)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2081 | ERN1 | endoplasmic reticulum to nu... | NM_001433.5 | 99.1% | 25.2% | (many diffs) |
| 2 | human | 2081 | ERN1 | endoplasmic reticulum to nu... | XM_017024347.2 | 95% | 22.9% | (many diffs) |
| 3 | human | 2081 | ERN1 | endoplasmic reticulum to nu... | XM_017024348.2 | 91.7% | 19.6% | (many diffs) |
| 4 | mouse | 78943 | Ern1 | endoplasmic reticulum (ER) ... | NM_023913.2 | 87.9% | 24.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 854
- ORF length:
- 756
- Sequence:
-
1 AAGGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG CCGGCCCGGC GGCTGCTGCT 121 GCTGCTGACG CTGCTGCTGC CCGGCCTCGG GATTTTTGGA AGTACCAGCA CAGTGACGCT 181 TCCTGAAACC TTGTTGTTTG TGTCAACGCT GGATGGAAGT TTGCATGCTG TCAGCAAGAG 241 GACAGGCTCA ATCAAATGGA CTTTAAAAGA AGATCCAGTC CTGCAGGTCC CAACACATGT 301 GGAAGAGCCT GCCTTTCTCC CAGATCCTAA TGATGGCAGC CTGTATACGC TTGGAAGCAA 361 GAATAATGAA GGCCTGACGA AACTTCCTTT TACCATCCCA GAATTGGTGC AGGCATCCCC 421 ATGCCGAAGT TCAGATGGAA TCCTCTACAT GGGTAAAAAG CAGGACATCT GGTATGTTAT 481 TGACCTCCTG ACCGGAGAGA AGCAGCAGAC TTTGTCATCG GCCTTTGCAG ATAGTCTCTG 541 CCCATCAACC TCTCTTCTGT ATCTTGGGCG AACAGAATAC ACCATCACCA TGTACGACAC 601 CAAAACCCGA GAGCTCCGGT GGAATGCCAC CTACTTTGAC TATGCGGCCT CACTGCCTGA 661 GGACGACGTG GACTACAAGA TGTCCCACTT TGTGTCCAAT GGTGATGGGC TGGTGGTGAC 721 TGTGGACAGT GAATCTGGGG ACGTCCTGTG GATCCAAAAC TACGCCTCCC CTGTGGTGGC 781 CTTTTATGTC TGGCAGCGGG AGGGTCTGAG GAAGGTGATG CACATCAATG TCGCTGTGAG 841 ACCCTGCGCT ATCTGACCTT CATGTCTGGG GGAGGTGGGG GCGCATCACA AAGTGGAAGT 901 ACCCGTTCCC CAAAGAGaca gaggccaaga gcaagctgac gcccactctg tatgttggga 961 aatactctac cagcctctat gcctctccct caatggtaca cgagggggtt gctgtcgtgc 1021 cccgcggcag cacacttcct ttgctggaag ggccccagac tgatggcgtc accatcgggg 1081 acaaggggga gtgtgtgatc acgcccagca cggacgtcaa gtttgatccc ggactcaaaa 1141 gcaagaacaa gctcaactac ttgaggaatt actggcttct gataggacac catgaaaccc 1201 cactgtctgc gtctaccaag atgctggaga gatttcccaa caatctaccc aaacatcggg 1261 aaaatgtgat tcctgctgat tcagagaaaa agagctttga ggaagttatc aacctggttg 1321 accagacttc agaaaacgca cctaccaccg tgtctcggga tgtggaggag aagcccgccc 1381 atgcccctgc ccggcccgag gcccccgtgg actccatgct taaggacatg gctaccatca 1441 tcctgagcac cttcctgctg attggctggg tggccttcat catcacctat cccctgagca 1501 tgcatcagca gcagcagctc cagcaccagc agttccagaa ggaactggag aagatccagc 1561 tcctgcagca gcagcagcag cagctgccct tccacccacc tggagacacg gctcaggacg 1621 gcgagctcct ggacacgtct ggcccgtact cagagagctc gggcaccagc agccccagca 1681 cgtcccccag ggcctccaac cactcgctct gctccggcag ctctgcctcc aaggctggca 1741 gcagcccctc cctggaacaa gacgatggag atgaggaaac cagcgtggtg atagttggga 1801 aaatttcctt ctgtcccaag gatgtcctgg gccatggagc tgagggcaca attgtgtacc 1861 ggggcatgtt tgacaaccgc gacgtggccg tgaagaggat cctccccgag tgttttagct 1921 tcgcagaccg tgaggtccag ctgttgcgag aatcggatga gcacccgaac gtgatccgct 1981 acttctgcac ggagaaggac cggcaattcc agtacattgc catcgagctg tgtgcagcca 2041 ccctgcaaga gtatgtggag cagaaggact ttgcgcatct cggcctggag cccatcacct 2101 tgctgcagca gaccacctcg ggcctggccc acctccactc cctcaacatc gttcacagag 2161 acctaaagcc acacaacatc ctcatatcca tgcccaatgc acacggcaag atcaaggcca 2221 tgatctccga ctttggcctc tgcaAGAAGG TGGGCAGTGG GGCAAACCAA GTTTTCAGCC 2281 GCCCGATTTG GGGGGCCCTG GCACAGAAGG TTGGATCGTC CCAGAGATGC TGAGCGAAGA 2341 CTGTAAGGAG AACCCTACGT ACACGGTGGA CATCTTTTTT GCAGGTTGCG TCTTTTACTA 2401 CGTAATCTCT GAGGGCAGCC ACCTTTTTGG CAAGTCCCTG CAGCGGCAGG CCAACATCCT 2461 CCTGGGTGCC TGCAGCCTTG ACTGCTTGCA CCCAGAGAAG CACGAAGACG TCATTGCACG 2521 TGAATTGATA GAGAAGATGA TTGCGATGGA TCCTCAGAAA CGCCCCTCAG CGAAGCACGT 2581 GCTCAAACAC CCGTTCTTCT GGAGCCTAGA GAAGCAGCTC CAGTTCTTCC AGGACGTGAG 2641 CGACAGAATA GAAAAGGAAT CCCTGGATGG CCCGATCGTG AAGCAGTTAG AGAGAGGCGG 2701 GAGAGCCGTG GTGAAGATGG ACTGGCGGGA GAACATCACT GTCCCCCTCC AGACAGACCT 2761 GCGTAAATTC AGGACCTATA AAGGTGGTTC TGTCAGAGAT CTCCTCCGAG CCATGAGAAA 2821 TAAGAAGCAC CACTACCGGG AGCTGCCTGC AGAGGTGCGG GAGACGCTGG GGTCCCTCCC 2881 CGACGACTTC GTGTGCTACT TCACGTCTCG CTTCCCCCAC CTCCTCGCAC ACACCTACCG 2941 GGCCATGGAG CTGTGCAGCC ACGAGAGACT CTTCCAGCCC TACTACTTCC ACGAGCCCCC 3001 AGAGCCCCAG CCCCCAGTGA CTCCAGACGC CCTCTTGCCA ACTTTCTTGT ACAAAGTTGG 3061 CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA 3121 TAAAATCATT ATTGCCATCC CAGCCTGCAA T