Construct: ORF ccsbBroadEn_14661
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018946.1_s300c1, BRDN0000458242
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- GSK3A (2931)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2931 | GSK3A | glycogen synthase kinase 3 ... | NM_019884.3 | 99.7% | 49.8% | 707_708insC;727_728insG;759delC |
| 2 | human | 2931 | GSK3A | glycogen synthase kinase 3 ... | XR_001753673.1 | 54.8% | (many diffs) | |
| 3 | mouse | 606496 | Gsk3a | glycogen synthase kinase 3 ... | NM_001031667.1 | 88.8% | 47.9% | (many diffs) |
| 4 | mouse | 606496 | Gsk3a | glycogen synthase kinase 3 ... | XM_006540258.1 | 83.5% | 52.3% | (many diffs) |
| 5 | mouse | 606496 | Gsk3a | glycogen synthase kinase 3 ... | XM_006540259.3 | 77.1% | 35.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 860
- ORF length:
- 762
- Sequence:
-
1 CAAGGGTTTT TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG AGCGGCGGCG GGCCTTCGGG 121 AGGCGGCCCT GGGGGCTCGG GCAGGGCGCG GACTAGCTCG TTCGCGGAGC CCGGCGGCGG 181 AGGCGGAGGA GGCGGCGGCG GCCCCGGAGG CTCGGCCTCC GGCCCAGGCG GCACCGGCGG 241 CGGAAAGGCA TCTGTCGGGG CCATGGGTGG GGGCGTCGGG GCCTCGAGCT CCGGGGGTGG 301 ACCCGGCGGC AGCGGCGGAG GAGGCAGCGG AGGCCCCGGC GCAGGCACTA GCTTCCCGCC 361 GCCCGGGGTG AAGCTGGGCC GTGACAGCGG GAAGGTGACC ACAGTCGTAG CCACTCTAGG 421 CCAAGGCCCA GAGCGCTCCC AAGAAGTGGC TTACACGGAC ATCAAAGTGA TTGGCAATGG 481 CTCATTTGGG GTCGTGTACC AGGCACGGCT GGCAGAGACC AGGGAACTAG TCGCCATCAA 541 GAAGGTTCTC CAGGACAAGA GGTTCAAGAA CCGAGAGCTG CAGATCATGC GTAAGCTGGA 601 CCACTGCAAT ATTGTGAGGC TGAGATACTT TTTCTACTCC AGTGGCGAGA AGAAAGACGA 661 GCTTTACCTA AATCTGGTGC TGGAATATGT GCCCGAGACA GTGTACCGGG TGGCCCGCCA 721 CTTCACCAAG GCCAAGTTGA CCATCCCTAT CCTCTATGTC AAGGTGTACA TGTACCAGCT 781 CTTCCGCAGC TTGGCCTACA TCCACCTCCC AGGGCGTGTG TCACCGGCGA CATCAAGCCC 841 CAGAACCTGC TGGTGGACCT GACACTGCTG TCCTCAAGCT CTGCGATTTT GGCAGTGCAA 901 AGCAGTTGGT CCGAGGGGAG CCCAATGTCT CCTACATCTG TTCTCGCTAC TACCGGGCCC 961 CAGAGCTCAT CTTTGGAGCC ACTGATTACA CCTCATCCAT CGATGTTTGG TCAGCTGGCT 1021 GTGTACTGGC AGAGCTCCTC TTGGGCCAGC CCATCTTCCC TGGGGACAGT GGGGTGGACC 1081 AGCTGGTGGA GATCATCAAG GTGCTGGGAA CACCAACCCG GGAACAAATC CGAGAGATGA 1141 ACCCCAACTA CACGGAGTTC AAGTTCCCTC AGATTAAAGC TCACCCCTGG ACAAAGGTGT 1201 TCAAATCTCG AACGCCGCCA GAGGCCATCG CGCTCTGCTC TAGCCTGCTG GAGTACACCC 1261 CATCCTCAAG GCTCTCCCCA CTAGAGGCCT GTGCGCACAG CTTCTTTGAT GAACTGCGAT 1321 GTCTGGGAAC CCAGCTGCCT AACAACCGCC CACTTCCCCC TCTCTTCAAC TTCAGTGCTG 1381 GTGAACTCTC CATCCAACCG TCTCTCAACG CCATTCTCAT CCCTCCTCAC TTGAGGTCCC 1441 CAGCGGGCAC TACCACCCTC ACCCCGTCCT CACAAGCTTT AACTGAGACT CCGACCAGCT 1501 CAGACTGGCA GTCGACCGAT GCCACACCTA CCCTCACTAA CTCCTCCTTG CCAACTTTCT 1561 TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA 1621 CTATCAGTCA AAATAAAATC ATTATAGCCA TCCAGCGTTC AGACG