Construct: ORF ccsbBroadEn_14679

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019046.1_s300c1, BRDN0000458664
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
JAK1 (3716)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3716 JAK1 Janus kinase 1 NM_001320923.1 100% 100%
2 human 3716 JAK1 Janus kinase 1 NM_001321852.2 100% 100%
3 human 3716 JAK1 Janus kinase 1 NM_001321853.2 100% 100%
4 human 3716 JAK1 Janus kinase 1 NM_001321854.2 100% 100%
5 human 3716 JAK1 Janus kinase 1 NM_001321855.2 100% 100%
6 human 3716 JAK1 Janus kinase 1 NM_001321856.1 100% 100%
7 human 3716 JAK1 Janus kinase 1 NM_002227.4 100% 100%
8 human 3716 JAK1 Janus kinase 1 NM_001321857.2 99.9% 99.9% 1456_1457insAGC
9 mouse 16451 Jak1 Janus kinase 1 NM_146145.2 89.3% 94.8% (many diffs)
10 mouse 16451 Jak1 Janus kinase 1 XM_006502812.3 89.3% 94.8% (many diffs)
11 mouse 16451 Jak1 Janus kinase 1 XM_011240451.2 89.3% 94.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3531
ORF length:
3462
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcaccat gcagtatcta aatataaaag aggactgcaa tgccatggct ttctgtgcta
121aaatgaggag ctccaagaag actgaggtga acctggaggc ccctgagcca ggggtggaag
181tgatcttcta tctgtcggac agggagcccc tccggctggg cagtggagag tacacagcag
241aggaactgtg catcagggct gcacaggcat gccgtatctc tcctctttgt cacaacctct
301ttgccctgta tgacgagaac accaagctct ggtatgctcc aaatcgcacc atcaccgttg
361atgacaagat gtccctccgg ctccactacc ggatgaggtt ctatttcacc aattggcatg
421gaaccaacga caatgagcag tcagtgtggc gtcattctcc aaagaagcag aaaaatggct
481acgagaaaaa aaagattcca gatgcaaccc ctctccttga tgccagctca ctggagtatc
541tgtttgctca gggacagtat gatttggtga aatgcctggc tcctattcga gaccccaaga
601ccgagcagga tggacatgat attgagaacg agtgtctagg gatggctgtc ctggccatct
661cacactatgc catgatgaag aagatgcagt tgccagaact gcccaaggac atcagctaca
721agcgatatat tccagaaaca ttgaataagt ccatcagaca gaggaacctt ctcaccagga
781tgcggataaa taatgttttc aaggatttcc taaaggaatt taacaacaag accatttgtg
841acagcagcgt gtccacgcat gacctgaagg tgaaatactt ggctaccttg gaaactttga
901caaaacatta cggtgctgaa atatttgaga cttccatgtt actgatttca tcagaaaatg
961agatgaattg gtttcattcg aatgacggtg gaaacgttct ctactacgaa gtgatggtga
1021ctgggaatct tggaatccag tggaggcata aaccaaatgt tgtttctgtt gaaaaggaaa
1081aaaataaact gaagcggaaa aaactggaaa ataaacacaa gaaggatgag gagaaaaaca
1141agatccggga agagtggaac aatttttctt acttccctga aatcactcac attgtaataa
1201aggagtctgt ggtcagcatt aacaagcagg acaacaagaa aatggaactg aagctctctt
1261cccacgagga ggccttgtcc tttgtgtccc tggtagatgg ctacttccgg ctcacagcag
1321atgcccatca ttacctctgc accgacgtgg cccccccgtt gatcgtccac aacatacaga
1381atggctgtca tggtccaatc tgtacagaat acgccatcaa taaattgcgg caagaaggaa
1441gcgaggaggg gatgtacgtg ctgaggtgga gctgcaccga ctttgacaac atcctcatga
1501ccgtcacctg ctttgagaag tctgagcagg tgcagggtgc ccagaagcag ttcaagaact
1561ttcagatcga ggtgcagaag ggccgctaca gtctgcacgg ttcggaccgc agcttcccca
1621gcttgggaga cctcatgagc cacctcaaga agcagatcct gcgcacggat aacatcagct
1681tcatgctaaa acgctgctgc cagcccaagc cccgagaaat ctccaacctg ctggtggcta
1741ctaagaaagc ccaggagtgg cagcccgtct accccatgag ccagctgagt ttcgatcgga
1801tcctcaagaa ggatctggtg cagggcgagc accttgggag aggcacgaga acacacatct
1861attctgggac cctgatggat tacaaggatg acgaaggaac ttctgaagag aagaagataa
1921aagtgatcct caaagtctta gaccccagcc acagggatat ttccctggcc ttcttcgagg
1981cagccagcat gatgagacag gtctcccaca aacacatcgt gtacctctat ggcgtctgtg
2041tccgcgacgt ggagaatatc atggtggaag agtttgtgga agggggtcct ctggatctct
2101tcatgcaccg gaaaagcgat gtccttacca caccatggaa attcaaagtt gccaaacagc
2161tggccagtgc cctgagctac ttggaggata aagacctggt ccatggaaat gtgtgtacta
2221aaaacctcct cctggcccgt gagggcatcg acagtgagtg tggcccattc atcaagctca
2281gtgaccccgg catccccatt acggtgctgt ctaggcaaga atgcattgaa cgaatcccat
2341ggattgctcc tgagtgtgtt gaggactcca agaacctgag tgtggctgct gacaagtgga
2401gctttggaac cacgctctgg gaaatctgct acaatggcga gatccccttg aaagacaaga
2461cgctgattga gaaagagaga ttctatgaaa gccggtgcag gccagtgaca ccatcatgta
2521aggagctggc tgacctcatg acccgctgca tgaactatga ccccaatcag aggcctttct
2581tccgagccat catgagagac attaataagc ttgaagagca gaatccagat attgtttcag
2641aaaaaaaacc agcaactgaa gtggacccca cacattttga aaagcgcttc ctaaagagga
2701tccgtgactt gggagagggc cactttggga aggttgagct ctgcaggtat gaccccgaag
2761gggacaatac aggggagcag gtggctgtta aatctctgaa gcctgagagt ggaggtaacc
2821acatagctga tctgaaaaag gaaatcgaga tcttaaggaa cctctatcat gagaacattg
2881tgaagtacaa aggaatctgc acagaagacg gaggaaatgg tattaagctc atcatggaat
2941ttctgccttc gggaagcctt aaggaatatc ttccaaagaa taagaacaaa ataaacctca
3001aacagcagct aaaatatgcc gttcagattt gtaaggggat ggactatttg ggttctcggc
3061aatacgttca ccgggacttg gcagcaagaa atgtccttgt tgagagtgaa caccaagtga
3121aaattggaga cttcggttta accaaagcaa ttgaaaccga taaggagtat tacaccgtca
3181aggatgaccg ggacagccct gtgttttggt atgctccaga atgtttaatg caatctaaat
3241tttatattgc ctctgacgtc tggtcttttg gagtcactct gcatgagctg ctgacttact
3301gtgattcaga ttctagtccc atggctttgt tcctgaaaat gataggccca acccatggcc
3361agatgacagt cacaagactt gtgaatacgt taaaagaagg aaaacgcctg ccgtgcccac
3421ctaactgtcc agatgaggtt tatcaactta tgaggaaatg ctgggaattc caaccatcca
3481atcggacaag ctttcagaac cttattgaag gatttgaagc acttttaaaa ttgccaactt
3541tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaac

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