Construct: ORF ccsbBroadEn_14799
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018934.1_s300c1, BRDN0000458230
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAPK1 (5594)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5594 | MAPK1 | mitogen-activated protein k... | NM_002745.4 | 99.4% | 63% | (many diffs) |
| 2 | human | 5594 | MAPK1 | mitogen-activated protein k... | NM_138957.3 | 99.4% | 63% | (many diffs) |
| 3 | mouse | 26413 | Mapk1 | mitogen-activated protein k... | NM_001038663.1 | 92.3% | 62.3% | (many diffs) |
| 4 | mouse | 26413 | Mapk1 | mitogen-activated protein k... | NM_011949.3 | 92.3% | 62.3% | (many diffs) |
| 5 | mouse | 26413 | Mapk1 | mitogen-activated protein k... | XM_006522147.3 | 92.3% | 62.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 100
- ORF end:
- 799
- ORF length:
- 699
- Sequence:
-
1 CAATGGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TGAGCAATGC 61 TTTTTTATAA TGCCAACTTT GTACAAAAAA GTTGGCACCA TGGCGGCGGC GGCGGCGGGC 121 GCGGGCCCGG AGATGGTCCG CGGGCAGGTG TTCGACGTGG GGCCGCGCTA CACCAACCTC 181 TCGTACATCG GCGAGGGCGC CTACGGCATG GTGTGCTCTG CTTATGATAA TGTCAACAAA 241 GTTCGAGTAG CTATCAAGAA AATCAGCCCC TTTGAGCACC AGACCTACTG CCAGAGAACC 301 CTGAGGGAGA TAAAAATCTT ACTGCGCTTC AGACATGAGA ACATCATTGG AATCAATGAC 361 ATTATTCGAG CACCAACCAT CGAGCAAATG AAAGATGTAT ATATAGTACA GGACCTCATG 421 GAAACAGATC TTTACAAGCT CTTGAAGACA CAACACCTCA GCAATGACCA TATCTGCTAT 481 TTTCTCTACC AGATCCTCAG AGGGTTAAAA TATATCCATT CAGCTAACGT TCTGCACCGT 541 GACCTCAAGC CTTCCAACNT GCTGCTCAAC ACCACCTGTG ATCTCAAGAT CTGTGACTTT 601 GGCNTGGCCC GTGTTGCAGA TCCAGACCAT GATCACACAG GGTTCCTGAC AGAATATGTG 661 GCCACACGTT GGTACAGGGC TCCAGAAATT ATGTTGAATT CCAAGGGCTA CACCAAGTCC 721 ATTGATATTT GGTCTGTAGG CTGCATTCTG GCAGAAATGC TTTCTAACAG GCCCATCTTT 781 CCAGGGAAAG CATTATCTTG ACCAGCTGAA CCACATTTTG GGTATTCTTG GATCCCCATC 841 ACAAGAAGAC CTGAATTGTA TAATAAATTT AAAAGCTAGG AACTATTTGC TTTCTCTTCC 901 ACACAAAAAT AAGGTGCCAT GGAACAGGCT GTTCCCAAAT GCTGACTCCA AAGCTCTGGA 961 CTTATTGGAC AAAATGTTGA CATTCAACCC ACACAAGAGG ATTGAAGTAG AACAGGCTCT 1021 GGCCCACCCA TATCTGGAGC AGTATTACGA CCCGAGTGAC GAGCCCATCG CCGAAGCACC 1081 ATTCAAGTTC GACATGGAAT TGGATGACTT GCCTAAGGAA AAGCTCAAAG AACTAATTTT 1141 TGAAGAGACT GCTAGATTCC AGCCAGGATA CAGATCTTTG CCAACTTTCT TGTACAAAGT 1201 TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA 1261 AAATAAAATC ATTATTGCCA TCCAGCGCGG CGTG