Construct: ORF ccsbBroadEn_14800
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018945.1_s300c1, BRDN0000458241
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAPK3 (5595)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5595 | MAPK3 | mitogen-activated protein k... | NM_002746.3 | 99.6% | 35.3% | (many diffs) |
| 2 | human | 5595 | MAPK3 | mitogen-activated protein k... | NM_001040056.3 | 92.5% | 37.5% | (many diffs) |
| 3 | human | 5595 | MAPK3 | mitogen-activated protein k... | NM_001109891.1 | 88% | 39.9% | (many diffs) |
| 4 | human | 5595 | MAPK3 | mitogen-activated protein k... | XR_243293.1 | 58.8% | (many diffs) | |
| 5 | mouse | 26417 | Mapk3 | mitogen-activated protein k... | NM_011952.2 | 89.6% | 33.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 97
- ORF end:
- 517
- ORF length:
- 420
- Sequence:
-
1 CATGGTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT 61 TTTATAATGC CAACTTTGTA CAAAAAAGTT GGCACCATGG CGGCGGCGGC GGCTCAGGGG 121 GGCGGGGGCG GGGAGCCCCG TAGAACCGAG GGGGTCGGCC CGGGGGTCCC GGGGGAGGTG 181 GAGATGGTGA AGGGGCAGCC GTTCGACGTG GGCCCGCGCT ACACGCAGTT GCAGTACATC 241 GGCGAGGGCG CGTACGGCAT GGTCAGCTCG GCCTATGACC ACGTGCGCAA GACTCGCGTG 301 GCCATCAAGA AGATCAGCCC CTTCGAACAT CAGACCTACT GCCAGCGCAC GCTCCGGGAG 361 ATCCAGATCC TGCTGCGCTT CCGCCATGAG AATGTCATCG GCATCCGAGA CATTCTGCGG 421 GCGTCCACCC TGGAAGCCAT GAGAGATGTC TACATTGTGC AGGACCTGAT GGAGACTGAC 481 CTGTACAAGT TGCTGAAAAG GCCAGCAGCT GAGCAATGAC CATAATCTGC TACTTCCTCT 541 ACCAGATCCT GCGGGGCCTC AAGTACATCC ACTCCGCCAA CGTGCTCCAC CGAGATCTAA 601 AGCCCTCCAA CCTGCTCATC AACACCACNT GCGACNTTAA GATTTGTGAT TTCGGCCTGG 661 CCCGGATTGC CGATCCTGAG CATGACCACA CCGGCTTCCT GACGGAGTAT GTGGCTACGC 721 GCTGGTACCG GGCCCCAGAG ATCATGCTGA ACTCCAAGGG CTATACCAAG TCCATCGACA 781 TCTGGTCTGT GGGCTGCATT CTGGCTGAGA TGCTCTCTAA CCGGCCCATC TTCCCTGGCA 841 AGCACTACCT GGATCAGCTC AACCACATTC TGGGCATCCT GGGCTCCCCA TCCCAGGAGG 901 ACCTGAATTG TATCATCAAC ATGAAGGCCC GAAACTACCT ACAGTCTCTG CCCTCCAAGA 961 CCAAGGTGGC TTGGGCCAAG CTTTTCCCCA AGTCAGACTC CAAAGCCCTT GACCTGCTGG 1021 ACCGGATGTT AACCTTTAAC CCCAATAAAC GGATCACAGT GGAGGAAGCG CTGGCTCACC 1081 CCTACCTGGA GCAGTACTAT GACCCGACGG ATGAGCCAGT GGCCGAGGAG CCCTTCACCT 1141 TCGCCATGGA GCTGGATGAC CTACCTAAGG AGCGGCTGAA GGAGCTCATC TTCCAGGAGA 1201 CAGCACGCTT CCAGCCCGGA GTGCTGGAGG CCCCCTTGCC AACTTTCTTG TACAAAGTTG 1261 GCATTATAAG AAAGCATTGC TTATCAATAT GTTGCAACGA ACAGGTCACT ATCAGTCAAA 1321 ATAAAATCAT TATTGCCATC CAGCTGCTCC AGG