Construct: ORF ccsbBroadEn_14813
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018819.1_s300c1, BRDN0000458115
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKX (5613)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5613 | PRKX | protein kinase X-linked | NM_005044.5 | 99.7% | 31.2% | 128T>C;249C>T;336_337insT |
| 2 | human | 5616 | PRKY | protein kinase Y-linked (ps... | NR_028062.1 | 13.2% | (many diffs) | |
| 3 | human | 441733 | PRKXP1 | PRKX pseudogene 1 | NR_073405.1 | 7.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 449
- ORF length:
- 351
- Sequence:
-
1 TTGGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG GAGGCGCCCG GGCTGGCCCA 121 GGCGGCCGCG GCGGAGAGCG ACTCCCGCAA GGTGGCGGAG GAGACCCCCG ACGGGGCGCC 181 CGCGCTCTGC CCCAGCCCTG AGGCGCTGTC GCCGGAGCCG CCTGCGTACA GCCTGCAGGA 241 CTTTGACACG CTGGCCACCG TGGGCACTGG GACGTTCGGG CGGGTGCACC TGGTGAAGGA 301 GAAGACAGCC AAGCATTTCT TCGCCCTCAA GGTGATGAGC ATTCCTGACG TCATCCGCCT 361 AAAGCAGGAG CAACACGTAC ACAATGAGAA GTCTGTCCTG AAGGAAGTCA GCCACCCGTT 421 CCTCATCAGG CTGTTTCTGG ACGTGGCATG ACGAGCGCTT CCTCTACATG CTCATGGAGT 481 ACGTGCCGGG CGGCGAGCTC TTCAGCTACC TGCGCAACCG GGGGCGCTTC TCCAGCACCA 541 CGGGGCTCTT CTACTCTGCA GAGATCATCT GTGCCATCGA GTACCTGCAC TCCAAAGAGA 601 TCGTCTACAG GGACTTGAAG CCAGAGAACA TCCTGCTGGA TAGGGATGGC CACATTAAGC 661 TCACGGACTT TGGGTTCGCC AAGAAGCTGG TAGACAGGAC TTGGACCCTC TGTGGAACAC 721 CCGAGTACCT AGCCCCCGAA GTCATTCAGA GCAAGGGCCA CGGAAGGGCC GTGGACTGGT 781 GGGCCCTCGG CATCCTGATA TTCGAGATGC TTTCGGGGTT TCCTCCGTTT TTTGATGACA 841 ACCCGTTTGG CATTTATCAG AAAATTCTTG CAGGCAAAAT AGATTTCCCC AGACATTTGG 901 ATTTCCATGT AAAAGACCTC ATTAAGAAAC TGCTCGTGGT TGACAGAACA AGGCGATTAG 961 GAAACATGAA GAACGGGGCG AATGATGTGA AGCATCATCG GTGGTTCCGC TCCGTGGACT 1021 GGGAAGCTGT TCCGCAGAGA AAACTGAAGC CTCCCATCGT GCCCAAGATA GCTGGTGACG 1081 GCGACACTTC CAACTTCGAA ACTTACCCTG AGAATGACTG GGACACAGCC GCGCCCGTGC 1141 CGCAGAAGGA TTTAGAAATC TTCAAGAATT TCTTGCCAAC TTTCTTGTAC AAAGTTGGCA 1201 TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA 1261 AAATCATTAT TGCCATCCAG CGATCCGGG