Construct: ORF ccsbBroadEn_14814
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018942.1_s300c1, BRDN0000458238
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKY (5616)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5613 | PRKX | protein kinase X-linked | NM_005044.5 | 73.6% | 69.8% | (many diffs) |
| 2 | human | 5616 | PRKY | protein kinase Y-linked (ps... | NR_028062.1 | 11.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 930
- ORF length:
- 831
- Sequence:
-
1 CTAGGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT 61 TTTTTATAAT GCCAACTTTG TACAAAAAAG TTGGCACCAT GGAGGCGCCC GGGCCGGCCC 121 AGGCGGCCGC GGCGGAGAGC AACTCCCGAG AGGTGACGGA GGATGCCGCC GACTGGGCGC 181 CCGCGCTCTG CCCCAGCCCC GAGGCGCNGT CGCCGGAGGC GCCTGCCTAC CGCCTGCAGG 241 ACTGCGACGC GCTGGTCACC ATGGGCACTG GGACGTTCGG GCGGGTGCAC CTGGTGAAGG 301 AGAAGACAGC CAAGCATTTC TTCGCCCTCA AGGTGATGAG CATTCCCGAC GTCATCCGCC 361 GGAAGCAGGA GCAGCACGTG CACAATGAGA AGTCTGTCCT GAAGGAAGTC AGCCACCCGT 421 TCCTCATCAG GCTGTTCTGG ACGTGGCATG AGGAGCGCTT CCTCTACATG CTCATGGAGT 481 ATGTGCCGGG TGGCGAGCTC TTCAGCTACC TGCGCAACCG GGGGCACTTC TCCAGCACCA 541 CGGGGCTCTT CTACTCTGCG GAGATCATCT GTGCCATTGA GTACCTGCAC TCCAAGGAGA 601 TCGTCTACAG GGATTTGAAG CCGGAGAACA TCCTGCTGGA TAGGGATGGT CACATCAAGC 661 TCACGGACTT TGGGTTTGCC AAGAAGCTGG TAGACAGGAC TTGGACCCTC TGTGGAACAC 721 CCGAGTACCT AGCCCCCGAA GTCATTCAGA GCAAGGGCCA CGGAAGGNCC GTGGACTGGT 781 GGGCCCTCGG CATCCTGATA TTCGAGATGC TTTCGGGGTT TCCTCCATTT TTTGATGACA 841 ACCCGTTTGG CATTTATCAG AAAATTCTTG CAGGCAAACT ATATTTCCCC AGACATTTGG 901 ATTTCCATGT AAAAACGGGG CGAATGATGT TGCCAACTTT CTTGTACAAA GTTGGCATTA 961 TAAGAAAGCA TTGCAAAACA ATATGATGCA ACGCCCAGCT CACGATCTAG TCAAAATAAA 1021 ATCATTATTG CCATCCAGCT GATTCCGCG