Construct: ORF ccsbBroadEn_14852
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018899.1_s300c1, BRDN0000458195
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CDKL5 (6792)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6792 | CDKL5 | cyclin dependent kinase like 5 | NM_001323289.2 | 85.1% | 23.4% | (many diffs) |
| 2 | human | 6792 | CDKL5 | cyclin dependent kinase like 5 | NM_001037343.1 | 79.3% | 21.8% | (many diffs) |
| 3 | human | 6792 | CDKL5 | cyclin dependent kinase like 5 | NM_003159.2 | 79.3% | 21.8% | (many diffs) |
| 4 | mouse | 382253 | Cdkl5 | cyclin-dependent kinase-like 5 | NM_001024624.2 | 78% | 23.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 81
- ORF end:
- 759
- ORF length:
- 678
- Sequence:
-
1 TGATAGTGAC CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT 61 TGTACAAAAA AGTTGGCACC ATGAAGATTC CTAACATTGG TAATGTGATG AATAAATTTG 121 AGATCCTTGG GGTTGTAGGT GAAGGAGCCT ATGGAGTTGT ACTTAAATGC AGACACAAGG 181 AAACACATGA AATTGTGGCG ATCAAGAAAT TCAAGGACAG TGAAGAAAAT GAAGAAGTCA 241 AAGAAACGAC TTTACGAGAG CTTAAAATGC TTCGGACTCT CAAGCAGGAA AACATTGTGG 301 AGTTGAAGGA AGCATTTCGT CGGAGGGGAA AGTTGTACTT GGTGTTTGAG TATGTTGAAA 361 AAAATATGCT CGAATTGCTG GAAGAAATGC CAAATGGAGT TCCACCTGAG AAAGTAAAAA 421 GCTACATCTA TCAGCTAATC AAGGCTATTC ACTGGTGCCA TAAGAATGAT ATTGTCCATC 481 GAGATATAAA ACCAGAAAAT CTCTTAATCA GCCACAATGA TGTCCTAAAA CTGTGTGACT 541 TTGGTTTTGC TCGTAATCTG TCAGAAGGCA ATAATGCTAA TTACACAGAG TACGTTGCCA 601 CCAGATGGTA TCGGTCCCCA GAACTCTTAC TTGGCGCTCC CTATGGAAAG TCCGTGGACA 661 TGTGGTCGGT GGGCTGTATT CTTGGGGAGC TTAGCGATGG ACAGCCTTTA TTTCCTGGAG 721 AAAGTGAAAT TGACCAACTT TTTACTATTC AGAAGTGCTA GGACCACTTC CATCTGAGCA 781 GATGAGCTTT TCTACAGTAA TCTCGCTTCA TGGGCTCGGT TTCAGCTGTA CATCTCAGTC 841 TGGAAAGAGA TACTGATTTG ATAGTGTCTA CTGACTATGA GATTACTGAG TTGGAcccag 901 ctgacagata cttgacagaa cagtgtttga atcaccctac atttcaaacc cagagacttc 961 tggatcgttc tccttcaagg tcagcaaaaa gaaaacctta ccatgtggaa agcagcacat 1021 tgtctaatag aaaccaagcc ggcaaaagca ctgctttgca gtctcaccac agatctaaca 1081 gcaaggacat ccagaacctg agtgtaggcc tgccccgggc tgacgaaggt ctccctgcca 1141 atgaaagctt cctaaatgga aaccttgctg gagctagtct tagtccactg cacaccaaaa 1201 cctaccaagc aagcagccag cctgggtcta ccagcaaaga tctcaccaac aacaacatac 1261 cacaccttct tagcccaaaa gaagccaagt caaaaacaga gtttgatttt aatattgacc 1321 caaagccttc agaaggccca gggacaaagt acctcaagtc aaacagcaga tctcagcaga 1381 accgccactc attcatggaa agctctcaaa gcaaagctgg gacactgcag cccaatgaaa 1441 agcagagtcg gcatagctat attgacacaa ttccccagtc ctctaggagt ccctcctaca 1501 ggaccaaggc caaaagccat ggggcactga gtgactccaa gtctgtgagc aacctttctg 1561 aagccagggc ccaaattgcg gagcccagta ccagtaggta cttcccatct agctgcttag 1621 acttgaattc tcccaccagc ccaaccccca ccagacacag tgacacgaga actttgctca 1681 gcccttctgg aagaaataac cgaaatgagg gaacgctgga ctcacgtcga accacaacca 1741 gacattctTA GACGATGAGG AAATGAAGCT GCCGGAGCAC ATGACCAGTA GCCATTCCCC 1801 ATTCACTGTC TGCACTCACG AAATCTTTTT CNTATGGACT GGGCTACACC AGCCCCTTTT 1861 CTCCCCAGCA ACGTCCTCAT AGGCATTCTA TGTATGTGAC CCGTGACAAA GTGAGAGCCA 1921 AGGGCTTGGA TGGAAGCTTG AGCATAGGGC AAGGGATGGC AGCTAGAGCC AACAGCCTGC 1981 AACTCTTGTC ACCCCAGCCT GGAGAACAGC TCCCTCCAGA GATGACTGTG GCAAGATCTT 2041 CGGTCAAAGA GACCTCCAGA GAAGGCACCT CTTCCTTCCA TACACGCCAG AAGTCTGAGG 2101 GTGGAGTGTA TCATGACCCA CACTCTGATG ATGGCACAGC CCCCAAAGAA AATAGACACC 2161 TATACAATGA TCCTGTGCCA AGGAGAGTTG GTAGCTTTTA CAGAGTGCCA TCTCCACGTC 2221 CAGACAATTC TTTCCATGAA AATAATGTGT CAACTAGAGT TTCTTCTCTA CCATCAGAGA 2281 GCAGTTCTGG AACCAACCAC TCAAAAAGAC AACCAGCATT CGATCCATGG AAAAGTCCTG 2341 AAAATATTAG TCATTCAGAG CAACTCAAGG AAAAAGAGAA GCAAGGATTT TTCAGGTCAA 2401 TGAAAAAGAA AAAGAAGAAA TCTCAAACAG TACCCAATTC CGACAGCCCT GATCTTCTGA 2461 CGTTGCAGAA ATCCATTCAT TCTGCTAGCA CTCCAAGCAG CAGACCAAAG GAGTGGCGCC 2521 CCGAGAAGAT CTCAGATCTG CAGACCTTGC CAACTTTCTT GTACAAAGTT GGCATTATAA 2581 GAAAGCATTG CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA 2641 TTATTGCCAT CCAGCCTGAA TCCCCCCC