Construct: ORF ccsbBroadEn_14943
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018971.1_s300c1, BRDN0000458267
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- IKBKE (9641)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9641 | IKBKE | inhibitor of nuclear factor... | NM_014002.4 | 99.2% | 28.6% | (many diffs) |
| 2 | human | 9641 | IKBKE | inhibitor of nuclear factor... | XM_005273356.2 | 99.2% | 28.6% | (many diffs) |
| 3 | human | 9641 | IKBKE | inhibitor of nuclear factor... | NM_001193322.2 | 90.9% | 31.2% | (many diffs) |
| 4 | human | 9641 | IKBKE | inhibitor of nuclear factor... | NM_001193321.2 | 87.4% | 17% | (many diffs) |
| 5 | human | 9641 | IKBKE | inhibitor of nuclear factor... | XM_017002868.1 | 82.2% | 12% | (many diffs) |
| 6 | human | 9641 | IKBKE | inhibitor of nuclear factor... | XR_922002.2 | 65.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 96
- ORF end:
- 867
- ORF length:
- 771
- Sequence:
-
1 AAAGGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT 61 TTTTTATAAT GCCAACTTTG TACAAAAAAG TTGGCATGCA GAGCACAGCC AATTACCTGT 121 GGCACACAGA TGACCTGCTG GGGCAGGGGG CCACTGCCAG TGTGTACAAG GCCCGCAACA 181 AGAAATCCGG AGAGCTGGTT GCTGTGAAGG TCTTCAACAC TACCAGCTAC CTGCGGCCCC 241 GCGAGGTGCA GGTGAGGGAG TTTGAGGTCC TGCGGAAGCT GAACCACCAG AACATCGTCA 301 AGCTCTTTGC GGTGGAGGAG ACGGGCGGAA GCCGGCAGAA GGTACTGGTG ATGGAGTACT 361 GCTCCAGTGG GAGCCTGCTG AGTGTGCTGG AGAGCCCTGA GAATGCCTTT GGGCTGCCTG 421 AGGATGAGTT CCTGGTGGTG CTGCGCTGTG TGGTGGCCGG CATGAACCAC CTGCGGGAGA 481 ACGGCATTGT GCATCGCGAC ATCAAGCCGG GGAACATCAT GCGCCTCGTA GGGGAGGAGG 541 GGCAGAGCAT CTACAAGCTG ACAGACTTCG GCGCTGCCCG GGAGCTGGAT GATGATGAGA 601 AGTTCGTCTC GGTCTATGGG ACTGAGGAGT ACCTGCATCC CGACATGTAT GAGCGGGGCG 661 GTGCTTCGAA AGCCCCAGCA AAAAGCGTTC GGGGTGACTG TGGATCTCTG GAGCATTGGA 721 GTGACCTTGT ACCATGCAGC CACTGGCAGC CTGCCCTTCA TCCCCTTTGG TGGGCCACGG 781 CGGAACAAGG AGATCATGTA CCGGATCACA CGGAGAAGCC GGCTGGGGCC ATTGCAGTGC 841 CCAGAGGCGG GAGACGGCCC CTGGAGTGAG CTACACCCTC CCCATCACCT GCCAGCTGTC 901 ACTGgggctg cagagccagc tggtgcccat cctggccaac atcctggagg tggagcaggc 961 caagtgctgg ggcttcgacc agttctttgc ggagaccagt gacatcctgc agcgagttgt 1021 cgtccatgtc ttctccctgt cccaggcagt cctgcaccac atctatatcc atgcccacaa 1081 cacgatagcc attttccagg aggccgtgca caagcagacc agtgtggccc cccgacacca 1141 ggagtacctc tttgagggtc acctctgtgt cctcgagccc agcgtctcag cacagcacat 1201 cgcccacacg acggcaagca gccccctgac cctcttcagc acagccatcc ctaaggggct 1261 ggccttcagg gaccctgctc tggacgtccc caagttcgtc cccaaagtgg acctgcaggc 1321 ggattacaac actgccaagg gcgtgttggg cgccggctac caggccctgc ggctggcacg 1381 ggccctgctg gatgggcagg agctaatgtt tcgggggctg cacGGGGTCA TGGAGGTGCT 1441 CCAGGCCCAC ATGCAGACCG ACTCTGGAAG TGGCAAGGAC ATCCCTCCTT TTTCCCTCAG 1501 CAGCACCTGG GAACTGAGAG GTTCAGCAGC GTGGCTGGAA CGCNTGAGAT CCAGGAACTG 1561 AAGGCGGCTG CAGAACTGAG GTCCAGGCTG CGGACTCTAG CGGAGGTCCT CTCCAGATGC 1621 TCCCAAAATA TCACGGAGAC CCAGGAGAGC CTGAGCAGCC TGAACCGGGA GCTGGTGAAG 1681 AGCCGGGATC AGGTACATGA GGACAGAAGC ATCCAGCAGA TTCAGTGCTG TTTGGACAAG 1741 ATGAACTTCA TCTACAAACA GTTCAAGAAG TCTAGGATGA GGCCAGGGCT TGGCTACAAC 1801 GAGGAGCAGA TTCACAAGCT GGATAAGGTG AATTTCAGTC ATTTAGCCAA AAGACTCCTG 1861 CAGGTGTTCC AGGAGGAGTG CGTGCAGAAG TATCAAGCGT CCTTAGTCAC ACACGGCAAG 1921 AGGATGAGGG TGGTGCACGA GACCAGGAAC CACCTGCGCC TGGTTGGCTG TTCTGTGGCT 1981 GCCTGTAACA CAGAAGCCCA GGGGGTCCAG GAGAGTCTCA GCAAGCTCCT GGAAGAGCTA 2041 TCTCACCAGC TCCTTCAGGA CCGAGCAAAG GGGGCTCAGG CCTCGCCGCC TCCCATAGCT 2101 CCTTACCCCA GCCCTACACG AAAGGACCTG CTTCTCCACA TGCAAGAGCT CTGCGAGGGG 2161 ATGAAGCTGC TGGCATCTGA CCTCCTGGAC AACAACCGCA TCATCGAACG GCTAAATAGA 2221 GTCCCAGCAC CTCCTGATGT CTTGCCAACT TTCTTGTACA AAGTTGGCAT TATAAGAAAG 2281 CATTGCTTAT CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT 2341 GCCATCCAGC CTGAATCCCC C