Transcript: Human NM_014002.4

Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-16
Taxon:
Homo sapiens (human)
Gene:
IKBKE (9641)
Length:
3240
CDS:
346..2496

Additional Resources:

NCBI RefSeq record:
NM_014002.4
NBCI Gene record:
IKBKE (9641)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148905 AGCATCCCGACATGTATGAG pXPR_003 CGG 555 26% 7 0.852 IKBKE IKBKE 77769
2 BRDN0001144791 TCAACACTACCAGCTACCTG pXPR_003 CGG 135 6% 4 0.6049 IKBKE IKBKE 77772
3 BRDN0001147958 CGTGCACAAGCAGACCAGTG pXPR_003 TGG 1030 48% 10 0.4289 IKBKE IKBKE 77770
4 BRDN0001148875 TGCATCGCGACATCAAGCCG pXPR_003 GGG 411 19% 6 0.0573 IKBKE IKBKE 77771
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_014002.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199922 GCATTGTGCATCGCGACATCA pLKO.1 734 CDS 100% 4.950 6.930 N IKBKE n/a
2 TRCN0000010036 TGGGCAGGAGCTAATGTTTCG pLKO.1 1647 CDS 100% 4.050 5.670 N IKBKE n/a
3 TRCN0000010035 TGCCCACAACACGATAGCCAT pLKO.1 1326 CDS 100% 2.640 3.696 N IKBKE n/a
4 TRCN0000010034 CTTCGACCAGTTCTTTGCGGA pLKO.1 1227 CDS 100% 0.660 0.924 N IKBKE n/a
5 TRCN0000234679 AGTCCTGCACCACATCTATAT pLKO_005 1302 CDS 100% 13.200 10.560 N IKBKE n/a
6 TRCN0000234681 GCATCATCGAACGGCTAAATA pLKO_005 2450 CDS 100% 15.000 10.500 N IKBKE n/a
7 TRCN0000234682 TCCCACTCCCTCTGGTTTATA pLKO_005 2831 3UTR 100% 15.000 10.500 N IKBKE n/a
8 TRCN0000234680 CAAGCTGGATAAGGTGAATTT pLKO_005 2067 CDS 100% 13.200 9.240 N IKBKE n/a
9 TRCN0000234678 AGAAGTTCGTCTCGGTCTATG pLKO_005 848 CDS 100% 10.800 7.560 N IKBKE n/a
10 TRCN0000195107 CCTGAAGCATTAGAATGATTC pLKO.1 2520 3UTR 100% 10.800 7.560 N IKBKE n/a
11 TRCN0000010027 GAGCATTGGAGTGACCTTGTA pLKO.1 960 CDS 100% 4.950 3.465 N IKBKE n/a
12 TRCN0000010037 GTCCTTAGTCACACACGGCAA pLKO.1 2151 CDS 100% 2.160 1.512 N IKBKE n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_014002.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000487809 ACCGGCGGAAATGCTTGCAGCGTC pLX_317 13.8% 99.9% 100% V5 (not translated due to prior stop codon) 201T>C n/a
2 ccsbBroadEn_14943 pDONR223 76% 99.2% 28.6% None (many diffs) n/a
3 ccsbBroad304_14943 pLX_304 27.7% 99.2% 28.6% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000467012 GACCTTCGTGTCCCGTTGGGCACG pLX_317 16.1% 99.2% 28.6% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_02218 pDONR223 100% 88.1% 88.1% None 1_255del n/a
6 ccsbBroad304_02218 pLX_304 31.3% 88.1% 88.1% V5 1_255del n/a
7 TRCN0000471250 CTATGAAATCCTCCGTGAGACTTA pLX_317 18.2% 88.1% 88.1% V5 1_255del n/a
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